蛋白质中的遗传变异：一维与二维凝胶电泳的比较
Genetic variation in proteins: comparison of one-dimensional and two-dimensional gel electrophoresis.
作者信息
McLellan T, Ames G F, Nikaido K
出版信息
Genetics. 1983 Jun;104(2):381-90. doi: 10.1093/genetics/104.2.381.
Abstract
Two proteins with known characteristics on one-dimensional gels were studied by two-dimensional electrophoresis to compare the sensitivities of the two methods in detecting genetic variation. Two-dimensional electrophoresis was found to be less sensitive than several types of one-dimensional gels in distinguishing variants of both proteins. Denaturation of proteins in urea in the two-dimensional method makes it possible to distinguish closely related proteins that differ from each other by units of charge. Many more types of variation in protein sequences can be distinguished on one-dimensional gels in the absence of denaturants. The estimates of heterozygosity based on two-dimensional gels are lower than those based on other methods, at least in part, because of the limited types of sequence differences that can be detected on two-dimensional gels. The application of two-dimensional electrophoresis to the measurement of genetic variation and to the detection of new mutations should be made carefully, in view of the limited sensitivity of the method in finding differences in sequence.
摘要
通过二维电泳对一维凝胶上具有已知特征的两种蛋白质进行了研究，以比较这两种方法在检测遗传变异方面的灵敏度。结果发现，在区分这两种蛋白质的变体时，二维电泳的灵敏度低于几种类型的一维凝胶。二维方法中蛋白质在尿素中的变性使得区分电荷单位不同的密切相关蛋白质成为可能。在没有变性剂的情况下，一维凝胶上可以区分更多类型的蛋白质序列变异。基于二维凝胶的杂合度估计低于基于其他方法的估计，至少部分原因是二维凝胶上可检测到的序列差异类型有限。鉴于该方法在发现序列差异方面灵敏度有限，在将二维电泳应用于遗传变异测量和新突变检测时应谨慎。
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本文引用的文献
1
The stepwise mutation model: an experimental evaluation utilizing hemoglobin variants.逐步突变模型：利用血红蛋白变体进行的实验评估。
Genetics. 1980 Jan;94(1):185-201. doi: 10.1093/genetics/94.1.185.
2
Genetic heterozygosity in a natural population of Mus musculus assessed using two-dimensional electrophoresis.使用二维电泳法评估小家鼠自然种群中的遗传杂合性。
Nature. 1980 Feb 28;283(5750):855-7. doi: 10.1038/283855a0.
3
Genetic variability of proteins from plasma membranes and cytosols of mouse organs.小鼠器官质膜和胞质溶胶中蛋白质的遗传变异性。
Biochem Genet. 1981 Oct;19(9-10):859-70. doi: 10.1007/BF00504251.
4
Towards a total human protein map.迈向完整的人类蛋白质图谱。
Nature. 1981 Aug 6;292(5823):491-2. doi: 10.1038/292491a0.
5
Genic variation in abundant soluble proteins of Drosophila melanogaster and Drosophila pseudoobscura.黑腹果蝇和拟暗果蝇丰富可溶性蛋白质中的基因变异。
Genetics. 1982 Nov;102(3):437-53. doi: 10.1093/genetics/102.3.437.
6
Genetic control of human apolipoprotein E polymorphism: comparison of one- and two-dimensional techniques of isoprotein analysis.人类载脂蛋白E多态性的遗传控制：同型蛋白分析的一维与二维技术比较
Hum Genet. 1982;60(4):344-51. doi: 10.1007/BF00569216.
7
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Proc Natl Acad Sci U S A. 1981 Jun;78(6):3784-8. doi: 10.1073/pnas.78.6.3784.
8
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9
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10
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