van Bockstaele D R, Berneman Z N, Peetermans M E
Cytometry. 1986 Mar;7(2):217-20. doi: 10.1002/cyto.990070215.
We report on an easy and reliable method for the enumeration of the typical cells present in the blood, bone marrow, and spleen of patients with hairy cell leukemia. Samples of five patients were analyzed. In three patients, the hairy cells were very accurately followed during treatment with alpha-interferon, using right-angle light scatter to differentiate them from other leukocytes present in total blood. Excellent correlation was obtained with the microscopic hairy cell enumeration. Additional verification by cell sorting and immunofluorescence studies confirmed these findings.
我们报告了一种简便可靠的方法，用于对毛细胞白血病患者血液、骨髓和脾脏中的典型细胞进行计数。分析了5例患者的样本。在3例患者中，使用直角光散射将毛细胞与全血中存在的其他白细胞区分开来，从而在α-干扰素治疗期间非常准确地跟踪毛细胞。与显微镜下毛细胞计数获得了极好的相关性。通过细胞分选和免疫荧光研究进行的额外验证证实了这些发现。
