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X 染色体失活复激活过程中 X 染色体上调丢失的谱系特异性动力学。

Lineage-specific dynamics of loss of X upregulation during inactive-X reactivation.

机构信息

Chromatin, RNA and Genome Laboratory, Department of Developmental Biology and Genetics, Indian Institute of Science, Bangalore 560012, India.

Chromatin, RNA and Genome Laboratory, Department of Developmental Biology and Genetics, Indian Institute of Science, Bangalore 560012, India; Department of Bioengineering, Indian Institute of Science, Bangalore 560012, India; IISc Mathematics Initiative (IMI), Indian Institute of Science, Bangalore 560012, India.

出版信息

Stem Cell Reports. 2024 Nov 12;19(11):1564-1582. doi: 10.1016/j.stemcr.2024.10.001. Epub 2024 Oct 31.

DOI:10.1016/j.stemcr.2024.10.001
PMID:39486405
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11589478/
Abstract

In mammals, X chromosome dosage is balanced between sexes through the silencing of one X chromosome in females. Recent single-cell RNA sequencing analysis demonstrated that the inactivation of the X chromosome is accompanied by the upregulation of the active X chromosome (Xa) during mouse embryogenesis. Here, we have investigated if the reactivation of inactive-X (Xi) leads to the loss of Xa upregulation in different cellular or developmental contexts. We find that while Xi reactivation and loss of Xa upregulation are tightly coupled in mouse embryonic epiblast and induced pluripotent stem cells, that is not the case in germ cells. Moreover, we demonstrate that partial reactivation of Xi in mouse extra-embryonic endoderm stem cells and human B cells does not result in the loss of Xa upregulation. Finally, we have established a mathematical model for the transcriptional coordination of two X chromosomes. Together, we conclude that the reactivation of Xi is not always synchronized with the loss of Xa upregulation.

摘要

在哺乳动物中，通过雌性中一条 X 染色体的沉默来平衡 X 染色体的剂量。最近的单细胞 RNA 测序分析表明，在小鼠胚胎发生过程中，X 染色体的失活伴随着活性 X 染色体 (Xa) 的上调。在这里，我们研究了失活的 X 染色体 (Xi) 的重新激活是否会导致不同细胞或发育环境中 Xa 上调的丧失。我们发现，虽然 Xi 的重新激活和 Xa 上调的丧失在小鼠胚胎外胚层和诱导多能干细胞中紧密耦合，但在生殖细胞中并非如此。此外，我们证明，在小鼠胚胎外胚层干细胞和人类 B 细胞中，Xi 的部分重新激活不会导致 Xa 上调的丧失。最后，我们建立了一个用于两个 X 染色体转录协调的数学模型。综上所述，Xi 的重新激活并不总是与 Xa 上调的丧失同步。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/26b1b40d6fb8/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/55e94b4e47ea/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/d25f66bbbb93/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/0d2db5c5b1de/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/99dc8ff3a67a/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/f65a0334196c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/26b1b40d6fb8/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/55e94b4e47ea/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/d25f66bbbb93/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/0d2db5c5b1de/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/99dc8ff3a67a/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/f65a0334196c/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221b/11589478/26b1b40d6fb8/gr6.jpg

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本文引用的文献

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Curr Biol. 2022 Oct 24;32(20):4397-4410.e5. doi: 10.1016/j.cub.2022.08.059. Epub 2022 Sep 14.
2
Single-cell analysis reveals X upregulation is not global in pre-gastrulation embryos.单细胞分析显示，在原肠胚形成前的胚胎中，X染色体上调并非全局性的。
iScience. 2022 May 25;25(6):104465. doi: 10.1016/j.isci.2022.104465. eCollection 2022 Jun 17.
3
Controlled X-chromosome dynamics defines meiotic potential of female mouse in vitro germ cells.
控制性 X 染色体动力学决定了雌性小鼠体外生殖细胞的减数分裂潜能。
EMBO J. 2022 Jun 14;41(12):e109457. doi: 10.15252/embj.2021109457. Epub 2022 May 23.
4
Preventing erosion of X-chromosome inactivation in human embryonic stem cells.防止人类胚胎干细胞中 X 染色体失活的侵蚀。
Nat Commun. 2022 May 6;13(1):2516. doi: 10.1038/s41467-022-30259-x.
5
Elastic dosage compensation by X-chromosome upregulation.通过X染色体上调实现弹性剂量补偿。
Nat Commun. 2022 Apr 6;13(1):1854. doi: 10.1038/s41467-022-29414-1.
6
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