Wang Xuangang, Kong Xiangfu, Chen Zhentao, Li Hengshun, Tao Ze, Zhang Quanqi, Yu Haiyang
Laboratary of Tropical Marine Germplasm Resources and Breeding Engineering, Sanya Oceanographic Institution, Ocean University of China, 572000, Sanya, Hainan, China.
Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, 266003, Qingdao, Shandong, China.
Fish Shellfish Immunol. 2024 Dec;155:109999. doi: 10.1016/j.fsi.2024.109999. Epub 2024 Oct 31.
Sebastes schlegelii is an economically significant marine fish that faces serious threats from various pathogens. Edwardsiella piscicida is a pathogenic bacterium that primarily affects fish, including S. schlegelii, leading to severe disease. Although numerous reports have documented the transcriptome sequencing of various fish tissues in response to E. piscicida infection, studies focusing on specific cells remain scarce. In this study, S. schlegelii were infected by intraperitoneal injection of E. piscicida. Severe external clinical signs were observed in E. piscicida-infected S. schlegelii and pathological examination demonstrated structural damage of the head kidney following treatment with E. piscicida. Furthermore, macrophages were isolated from the head kidneys of both the control and E. piscicida-infected groups for RNA sequencing (RNA-seq). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the DEGs were closely associated with immune response and oxidative stress. Additionally, Weighted Gene Co-expression Network Analysis (WGCNA) was performed based on the data from this study and RNA-seq files of macrophages infected with E. piscicida in vitro, revealing that immune responses, oxidative stress, and mitochondrial damage were involved in the macrophage response to E. piscicida infection both in vivo and in vitro. This study provides a reference for understanding the mechanisms by which teleost immune cells respond to pathogen invasion and enhances our comprehension of teleost innate immunity.
许氏平鲉是一种具有重要经济价值的海洋鱼类，面临着来自各种病原体的严重威胁。杀鱼爱德华氏菌是一种主要影响鱼类（包括许氏平鲉）的病原菌，会导致严重疾病。尽管有许多报道记录了各种鱼类组织对杀鱼爱德华氏菌感染的转录组测序，但针对特定细胞的研究仍然很少。在本研究中，通过腹腔注射杀鱼爱德华氏菌感染许氏平鲉。在感染杀鱼爱德华氏菌的许氏平鲉中观察到严重的外部临床症状，病理检查表明用杀鱼爱德华氏菌处理后头肾出现结构损伤。此外，从对照组和感染杀鱼爱德华氏菌组的头肾中分离出巨噬细胞进行RNA测序（RNA-seq）。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，差异表达基因与免疫反应和氧化应激密切相关。此外，基于本研究的数据和体外感染杀鱼爱德华氏菌的巨噬细胞的RNA-seq文件进行了加权基因共表达网络分析（WGCNA），结果表明免疫反应、氧化应激和线粒体损伤在体内和体外巨噬细胞对杀鱼爱德华氏菌感染的反应中均有涉及。本研究为理解硬骨鱼免疫细胞对病原体入侵的反应机制提供了参考，并增强了我们对硬骨鱼先天免疫的理解。
