Choe Yunjeong, Yu Jong Earn, Park Junmo, Park Dongchul, Oh Jeong-Il, Kim Suhkmann, Moon Ki Hwan, Kang Ho Young
Department of Microbiology, College of Natural Sciences, Pusan National University, Busan, 46241, South Korea.
Department of Chemistry, College of Natural Sciences, Pusan National University, Busan, 46241, South Korea.
Vet Res Commun. 2017 Dec;41(4):289-297. doi: 10.1007/s11259-017-9700-2. Epub 2017 Nov 8.
This study demonstrates the feasibility of using goldfish as an infection model to investigate the pathogenesis of Edwardsiella piscicida. Goldfish were found to be susceptible to acute E. piscicida-induced disease and died in a dose-dependent manner. E. piscicida was further shown to replicate rapidly in the head kidneys and livers of infected goldfish from 1 d post-injection, and bacteria numbers were significantly decreased 5 d post-injection. Immune responses were successfully induced in goldfish injected with E. piscicida strains and 60% of goldfish inoculated with an attenuated E. piscicida strain were found to survive subsequent injection with a pathogenic strain. The results of differential leukocyte count experiments suggested that leukocytes were immediately recruited as an innate immune response against the infection. Thus, this well-characterized goldfish species is a suitable infection model for studying E. piscicida pathogenesis, and might be applicable to research on other fish diseases.
本研究证明了使用金鱼作为感染模型来研究杀鱼爱德华氏菌发病机制的可行性。发现金鱼对杀鱼爱德华氏菌诱导的急性疾病敏感，并呈剂量依赖性死亡。进一步研究表明，杀鱼爱德华氏菌在注射后1天开始在受感染金鱼的头肾和肝脏中迅速繁殖，而在注射后5天细菌数量显著减少。用杀鱼爱德华氏菌菌株注射的金鱼成功诱导了免疫反应，并且发现接种减毒杀鱼爱德华氏菌菌株的金鱼中有60%在随后注射致病菌株后存活下来。白细胞分类计数实验结果表明，白细胞作为针对感染的固有免疫反应被立即募集。因此，这种特征明确的金鱼品种是研究杀鱼爱德华氏菌发病机制的合适感染模型，并且可能适用于其他鱼类疾病的研究。
