铜四胺氟化物对牙本质龋的抗菌和矿化性能：一项实验室研究。
Antibacterial and mineralising properties of copper tetraamine fluoride on dentine caries: A laboratory study.
机构信息
Faculty of Dentistry, University of Hong Kong, Hong Kong, China.
Faculty of Dentistry, University of Hong Kong, Hong Kong, China; Department of Mineralized Tissue Biology and Bioengineering, The ADA Forsyth Institute, Cambridge, MA, USA.
出版信息
J Dent. 2024 Dec;151:105444. doi: 10.1016/j.jdent.2024.105444. Epub 2024 Oct 31.
OBJECTIVE
To investigate the antibacterial, mineralising, and discolouring properties of copper tetraamine fluoride (CTF) on artificial dentine caries.
METHOD
Demineralised human dentine blocks were treated with CTF, silver diamine fluoride (SDF, positive control) and water (negative control) before they were subjected to cariogenic challenge with Streptococcus mutans biofilm. The morphology, viability, and growth kinetics of the biofilm were assessed by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony-forming unit (CFU) counting. The lesion depths, mineral loss, chemical structure, and crystal characteristics were measured using micro-computed tomography, Fourier transform infrared (FTIR), and X-ray diffraction (XRD). The discolouring property on demineralised dentine was assessed by spectrophotometry. One-way analysis of variance with the Bonferroni post hoc test was performed to assess and compare the data.
RESULTS
SEM revealed confluent bacterial growth covering the surface of dentine treated with Water but not CTF and SDF. CLSM showed the dead-to-live ratio of the biofilm treated with CTF, SDF and Water were 0.7 ± 0.1, 0.8 ± 0.1 and 0.5 ± 0.1 (p < 0.001, CTF, SDF>Water). The Log CFU values of the biofilm treated with CTF, SDF and Water were 7.8 ± 0.1, 7.8 ± 0.1 and 8.1 ± 0.2 (p < 0.001, CTF, SDF<Water). Micro-computed tomography showed the Δlesion-depth (μm) of dentine treated with CTF, SDF and water were 33 ± 5, 40 ± 7 and 169 ± 17 (p < 0.001, CTF, SDF<Water). The Δmineral loss (gHApcm) of dentine treated with CTF, SDF and Water were 0.19 ± 0.04, 0.18 ± 0.04 and 0.30 ± 0.04 (p < 0.001, CTF, SDF<Water). FTIR showed the amide I-to-hydrogen phosphate ratios of dentine treated with CTF, SDF and Water were 0.23 ± 0.02, 0.24 ± 0.05, and 0.44 ± 0.06 (p < 0.001, CTF, SDF<Water). XRD revealed well-crystallised hydroxyapatite in the CTF and SDF groups. Spectrophotometry showed the ΔE values of the CTF, SDF and Water groups were 6 ± 4, 49 ± 3 and 10 ± 3 (p < 0.001, CTF, water<SDF).
CONCLUSION
CTF inhibited Streptococcus mutans. It mineralised but not discoloured artificial dentine caries.
CLINICAL SIGNIFICANCE
If CTF is successfully translated into clinical application, it may be a non-discolouring anti-caries agent for clinicians to arrest dentine caries.
目的
研究四胺铜氟化物（CTF）对人工牙本质龋的抗菌、矿化和变色性能。
方法
将脱矿的人牙本质块用 CTF、银胺氟化氢（SDF，阳性对照）和水（阴性对照）处理，然后用变异链球菌生物膜进行致龋性挑战。通过扫描电子显微镜（SEM）、共聚焦激光扫描显微镜（CLSM）和菌落形成单位（CFU）计数评估生物膜的形态、活力和生长动力学。使用微计算机断层扫描、傅立叶变换红外光谱（FTIR）和 X 射线衍射（XRD）测量病变深度、矿物质损失、化学结构和晶体特征。通过分光光度法评估脱矿牙本质的变色性能。采用单因素方差分析和 Bonferroni 事后检验对数据进行评估和比较。
结果
SEM 显示，用 Water 处理的牙本质表面有融合的细菌生长，但 CTF 和 SDF 没有。CLSM 显示，用 CTF、SDF 和 Water 处理的生物膜的死活比为 0.7±0.1、0.8±0.1 和 0.5±0.1（p<0.001，CTF、SDF>Water）。用 CTF、SDF 和 Water 处理的生物膜的 Log CFU 值分别为 7.8±0.1、7.8±0.1 和 8.1±0.2（p<0.001，CTF、SDF<Water）。微计算机断层扫描显示，用 CTF、SDF 和水处理的牙本质的 Δlesion-depth（μm）分别为 33±5、40±7 和 169±17（p<0.001，CTF、SDF<Water）。用 CTF、SDF 和水处理的牙本质的 Δmineral loss（gHApcm）分别为 0.19±0.04、0.18±0.04 和 0.30±0.04（p<0.001，CTF、SDF<Water）。FTIR 显示，用 CTF、SDF 和水处理的牙本质的酰胺 I 与磷酸氢盐比值分别为 0.23±0.02、0.24±0.05 和 0.44±0.06（p<0.001，CTF、SDF<Water）。XRD 显示 CTF 和 SDF 组中存在结晶良好的羟基磷灰石。分光光度法显示 CTF、SDF 和 Water 组的 ΔE 值分别为 6±4、49±3 和 10±3（p<0.001，CTF、water<SDF）。
结论
CTF 抑制变形链球菌。它矿化但不使人工牙本质龋变色。
临床意义
如果 CTF 成功转化为临床应用，它可能是一种非着色的抗龋剂，供临床医生用于阻止牙本质龋。
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