通过随机诱变研究一株阴沟肠杆菌中黏菌素异质性耐药的机制
Study of the mechanisms of colistin heteroresistance in a strain of Enterobacter cloacae by random mutagenesis.
作者信息
Milleville Romane, Hadjadj Linda, Rolain Jean-Marc, Baron Sophie Alexandra
机构信息
Aix Marseille University, MEPHI, Faculté de Médecine et de Pharmacie, Marseille, France; IHU Méditerranée Infection, Marseille, France.
IHU Méditerranée Infection, Marseille, France; Aix Marseille University, APHM, MEPHI, Faculté de Médecine et de Pharmacie, Marseille, France.
出版信息
J Glob Antimicrob Resist. 2024 Dec;39:247-249. doi: 10.1016/j.jgar.2024.10.260. Epub 2024 Oct 30.
OBJECTIVE
The Enterobacter cloacae complex belongs to a group in which colistin resistance is not well documented due to their frequent heteroresistance.
METHODS
We investigated the molecular basis of colistin heteroresistance using genome analysis and random mutagenesis in a strain of E. cloacae.
RESULTS
The arnA gene was truncated in the six colistin susceptible mutants we obtained. However, the mcr-9 gene was present in these mutants suggesting this gene is not responsible for colistin resistance.
CONCLUSIONS
Previous studies have highlighted the role of the two-component system PhoPQ and its negative regulator mgrB or the arnBCADTEF operon. Our results confirm the crucial role of this operon.
目的
阴沟肠杆菌复合体属于一类由于频繁出现异质性耐药而对黏菌素耐药情况记录不完善的菌群。
方法
我们利用基因组分析和随机诱变技术，对一株阴沟肠杆菌进行研究，以探究黏菌素异质性耐药的分子基础。
结果
在我们获得的6株对黏菌素敏感的突变体中，arnA基因发生了截短。然而，这些突变体中存在mcr-9基因，这表明该基因与黏菌素耐药无关。
结论
先前的研究强调了双组分系统PhoPQ及其负调控因子mgrB或arnBCADTEF操纵子的作用。我们的结果证实了该操纵子的关键作用。
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