Safarpour Rahim, Pooresmaeil Malihe, Namazi Hassan
Polymer Research Laboratory, Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Polymer Research Laboratory, Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran; Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Science, Tabriz, Iran.
Int J Biol Macromol. 2024 Dec;282(Pt 2):137096. doi: 10.1016/j.ijbiomac.2024.137096. Epub 2024 Oct 30.
Considering the benefits of controlled drug delivery in cancer treatment, as well as the importance of biological macromolecules in this area, herein, the pre-synthesized carboxymethyl starch (CMS) was converted to CMS nanoparticles (CMS NPs) in one easy nanoprecipitation way. Thereafter, the Ag@MOF(Ag) was in situ synthesized in the presence of pre-prepared CMS NPs (CMS NPs/Ag@MOF(Ag)). Eventually, the functionalization with folic acid (FA) obtained the CMS NPs/Ag@MOF(Ag)-FA. The success of the accomplished process was approved by doing several techniques, including FT-IR, XRD, EDX, AFM, etc. The SEM analysis showed a combination of rod-like and spherical-like morphology for the fabricated bio-nanocomposite. The generated CMS NPs/Ag@MOF(Ag)-FA with a surface area of 10.595 m/g displayed a pore size of 13.666 nm and 82.99 % of doxorubicin (DOX) loading efficiency (DOX@CMS NPs/Ag@MOF(Ag)-FA). The 38.46 % and 58.19 % of loaded DOX were released respectively within 240 h at pH 7.4 and pH 5.0, referring to the pH-responsivity of the constructed system. 27.25 % of inhibitory effects on HeLa cells were obtained for the drug-loaded bio-nanocomposite. The CMS NPs/Ag@MOF(Ag)-FA also displayed an inherent antibacterial activity towards two common gram-negative and gram-positive bacteria. All of these results can contribute to developing polysaccharide-based porous systems in controlled cancer therapy.
考虑到可控药物递送在癌症治疗中的益处，以及生物大分子在该领域的重要性，本文采用一种简单的纳米沉淀方法将预合成的羧甲基淀粉（CMS）转化为CMS纳米颗粒（CMS NPs）。此后，在预先制备的CMS NPs存在下原位合成Ag@MOF(Ag)（CMS NPs/Ag@MOF(Ag)）。最终，用叶酸（FA）进行功能化得到CMS NPs/Ag@MOF(Ag)-FA。通过傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）、能量散射X射线光谱（EDX）、原子力显微镜（AFM）等多种技术验证了上述过程的成功。扫描电子显微镜（SEM）分析表明，所制备的生物纳米复合材料具有棒状和球状形态的组合。所制备的CMS NPs/Ag@MOF(Ag)-FA比表面积为10.595 m/g，孔径为13.666 nm，阿霉素（DOX）负载效率为82.99%（DOX@CMS NPs/Ag@MOF(Ag)-FA）。由于构建体系具有pH响应性，负载的DOX在pH 7.4和pH 5.0条件下分别在240小时内释放了38.46%和58.19%。载药生物纳米复合材料对HeLa细胞的抑制作用为27.25%。CMS NPs/Ag@MOF(Ag)-FA对两种常见的革兰氏阴性菌和革兰氏阳性菌也具有固有抗菌活性。所有这些结果都有助于开发基于多糖的多孔体系用于可控癌症治疗。
