变应性真菌性鼻-鼻窦炎鼻息肉上皮中前列腺素E受体2（EP2）的失调
Prostaglandin E Receptor 2 (EP2) Dysregulation in Allergic Fungal Rhinosinusitis Nasal Polyp Epithelium.
作者信息
Smith-Davidson Prestina, Altartoor Khaled, Kabongo M M, Claussen Henry, Arthur Robert A, Johnston H R, DelGaudio John M, Wise Sarah K, Solares C A, Barrow Emily M, Magliocca Kelly R, Koval Michael, Levy Joshua M
机构信息
Sinonasal and Olfaction Program, National Institute on Deafness and Other Communication Disorders (NIDCD), NIH, Bethesda, Maryland, U.S.A.
Department of Otolaryngology - Head and Neck Surgery, Emory University School of Medicine, Emory University, Atlanta, Georgia, U.S.A.
出版信息
Laryngoscope. 2025 Apr;135 Suppl 1(Suppl 1):S1-S8. doi: 10.1002/lary.31868. Epub 2024 Nov 2.
OBJECTIVES
Allergic fungal rhinosinusitis (AFRS) is an eosinophilic subtype of chronic rhinosinusitis with nasal polyposis (CRSwNP). This study aimed to investigate the transcriptome of AFRS nasal polyp epithelium.
METHODS
Sinonasal epithelial cells were harvested from healthy nasal mucosa and polyp tissue collected from participants undergoing elective sinonasal surgery. Primary epithelial cells were subsequently grown in air/liquid interface and subjected to RNA-seq analysis, RT-qPCR, immunoblotting, and immunostaining.
RESULTS
A total of 19 genes were differentially expressed between healthy and AFRS sample epithelium. The second top candidate gene, ranked by adjusted p-value, was prostaglandin E receptor 2 (PTGER2). The upregulation of PTGER2 was confirmed by RT-qPCR and immunoblot. The presence of the EP2 receptor, encoded by the PTGER2 gene, was confirmed by immunocytochemistry.
CONCLUSION
PTGER2 is a potential novel therapeutic target for AFRS. EP2 dysregulation is associated with aspirin-exacerbated respiratory disease, potentially giving insight into common mechanisms of disease in severe CRSwNP.
LEVEL OF EVIDENCE
NA Laryngoscope, 135:S1-S8, 2025.
目的
变应性真菌性鼻-鼻窦炎（AFRS）是慢性鼻-鼻窦炎伴鼻息肉（CRSwNP）的一种嗜酸性粒细胞亚型。本研究旨在探究AFRS鼻息肉上皮的转录组。
方法
从接受择期鼻-鼻窦手术的参与者所采集的健康鼻黏膜和息肉组织中获取鼻-鼻窦上皮细胞。随后将原代上皮细胞在气液界面培养，并进行RNA测序分析、逆转录定量聚合酶链反应（RT-qPCR）、免疫印迹和免疫染色。
结果
健康样本上皮与AFRS样本上皮之间共有19个基因差异表达。按校正p值排名第二的候选基因是前列腺素E受体2（PTGER2）。RT-qPCR和免疫印迹证实了PTGER2的上调。免疫细胞化学证实了由PTGER2基因编码的EP2受体的存在。
结论
PTGER2是AFRS一个潜在的新型治疗靶点。EP2失调与阿司匹林加重的呼吸道疾病相关，这可能为严重CRSwNP的常见发病机制提供见解。
证据级别
无 喉镜，135:S1 - S8，2025年。
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