未孕母牛和母羊黄体中的神经垂体素：在大黄体细胞的膜结合分泌颗粒中的免疫细胞化学定位
Luteal neurophysin in the nonpregnant cow and ewe: immunocytochemical localization in membrane-bounded secretory granules of the large luteal cell.
作者信息
Fields M J, Fields P A
出版信息
Endocrinology. 1986 Apr;118(4):1723-5. doi: 10.1210/endo-118-4-1723.
PMID:3948797
Abstract
The highly conserved neurophysin associated with neurohypophysial hormones was localized in the large luteal cell of the nonpregnant cow and nonpregnant ewe with light microscopy avidin-biotin immunohistochemistry. Furthermore, with rabbit anti-oxytocin-neurophysin serum and goat anti-rabbit immunoglobulin G-colloidal gold, neurophysin was localized in the 100- to 200-nm diameter membrane-bounded secretory granules of the large luteal cells.
摘要
采用抗生物素蛋白-生物素免疫组织化学的光学显微镜技术，将与神经垂体激素相关的高度保守的神经垂体素定位于未孕母牛和未孕母羊的大黄体细胞中。此外，使用兔抗催产素-神经垂体素血清和山羊抗兔免疫球蛋白G-胶体金，将神经垂体素定位于大黄体细胞直径为100至200纳米的膜结合分泌颗粒中。
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