Khan-Dawood F S
Acta Endocrinol (Copenh). 1986 Dec;113(4):570-5. doi: 10.1530/acta.0.1130570.
Immunoreactive oxytocin is detectable in the corpora lutea of women and cynomolgus monkeys by radioimmunoassay. To localize the presence of oxytocin and neurophysin I in ovarian tissues of subhuman primates, three corpora lutea and ovarian stromal tissues and two Fallopian tubes obtained during the menstrual cycle of the baboon and decidua from two pregnant baboons were examined using highly specific antisera against either oxytocin or neurophysin I and peroxidase-antiperoxidase light microscopy immunohistochemistry. Oxytocin-like as well as neurophysin I-like immunoreactivities were found in some cells of all the corpora lutea only, but could not be demonstrated in ovarian stromal tissues, Fallopian tubes and decidua. Specificity of the immunocytochemical reaction was further confirmed by immunoabsorption of the antiserum with excess oxytocin or neurophysin, after which the immunoreactivities for both oxytocin and neurophysin in the luteal tissue were negative. Similar controls using normal rabbit serum gave no positive staining for either oxytocin or neurophysin. Counterstaining of the positive immunoreactivities for oxytocin and neurophysin I with Mayer's haematoxylin and eosin demonstrated clearly that the oxytocin and neurophysin I appeared as granular material mainly within the cytoplasm of the luteal cells. The localization of immunoreactive oxytocin and neurophysin I in the corpus luteum of the baboon demonstrates directly the presence of these two neurohypophysial peptides within primate luteal cells and suggests their local production.
通过放射免疫分析法可在女性和食蟹猴的黄体中检测到免疫反应性催产素。为了在非人灵长类动物的卵巢组织中定位催产素和神经垂体素I的存在，使用针对催产素或神经垂体素I的高度特异性抗血清以及过氧化物酶-抗过氧化物酶光镜免疫组织化学技术，对狒狒月经周期中获取的三个黄体、卵巢基质组织、两条输卵管以及两只怀孕狒狒的蜕膜进行了检查。仅在所有黄体的一些细胞中发现了催产素样以及神经垂体素I样免疫反应性，但在卵巢基质组织、输卵管和蜕膜中未得到证实。用过量的催产素或神经垂体素对抗血清进行免疫吸收，进一步证实了免疫细胞化学反应的特异性，之后黄体组织中催产素和神经垂体素的免疫反应性均为阴性。使用正常兔血清进行的类似对照实验，催产素或神经垂体素均未出现阳性染色。用梅耶苏木精和伊红对催产素和神经垂体素I的阳性免疫反应进行复染，清楚地表明催产素和神经垂体素I主要以颗粒物质的形式出现在黄体细胞的细胞质内。狒狒黄体中免疫反应性催产素和神经垂体素I的定位直接证明了这两种神经垂体肽在灵长类黄体细胞中的存在，并提示它们是在局部产生的。
