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一种用于检测秀丽隐杆线虫、小鼠和人类全血中NAD/NADH的基于发光的方法。

A luminescent-based protocol for NAD/NADH detection in C. elegans, mice, and human whole blood.

作者信息

Wang He-Ling, Zhang Jianying, Cao Shu-Qin, Lagartos-Donate Maria Jose, Zhang Shi-Qi, Lautrup Sofie, Hu Zeping, Lyssiotis Costas A, Houtkooper Riekelt H, Fang Evandro F

机构信息

Department of Clinical Molecular Biology, University of Oslo and Akershus University Hospital, 1478 Lørenskog, Norway.

Department of Clinical Molecular Biology, University of Oslo and Akershus University Hospital, 1478 Lørenskog, Norway; Xiangya School of Stomatology, Central South University, Changsha, Hu'nan 410083, China.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103428. doi: 10.1016/j.xpro.2024.103428. Epub 2024 Nov 1.

DOI:10.1016/j.xpro.2024.103428
PMID:39487980
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11567065/
Abstract

Here, we present a NAD/NADH detection assay for evaluating NAD, NADH, and NAD/NADH ratio across diverse biological models, including Caenorhabditis elegans, mouse muscle tissue, mouse whole blood, and human whole blood. We describe steps for sample collection and preparation from different models as well as detection and calculation of NAD and NADH levels. This protocol is applicable for quantifying cellular/tissue NAD and NADH levels across different biological models.

摘要

在此，我们介绍一种用于评估不同生物模型（包括秀丽隐杆线虫、小鼠肌肉组织、小鼠全血和人类全血）中烟酰胺腺嘌呤二核苷酸（NAD）、还原型烟酰胺腺嘌呤二核苷酸（NADH）以及NAD/NADH比值的检测方法。我们描述了从不同模型采集和制备样本的步骤，以及NAD和NADH水平的检测与计算方法。该方案适用于定量不同生物模型中细胞/组织的NAD和NADH水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/1697bc8d1839/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/3cd7db9ba7e9/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/2bcfe38d4c80/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/3388b8b0de3c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/85aed1b2de13/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/1697bc8d1839/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/3cd7db9ba7e9/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/2bcfe38d4c80/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/3388b8b0de3c/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/85aed1b2de13/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7f4/11567065/1697bc8d1839/gr4.jpg

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