• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

HT-29/PBMC共培养模型中肠道屏障功能和免疫相互作用的临床前评估方案。

Protocol for the preclinical evaluation of gut barrier function and immune interaction in an HT-29/PBMC co-culture model.

作者信息

Astre Gwendoline, Créchet Laurianne, Pomié Nicolas, Pereira Ophélie, Cani Patrice D, Knauf Claude, Abot Anne

机构信息

Enterosys SAS, Labège, France.

NeuroMicrobiota, International Research Program (IRP) INSERM/UCLouvain, France/Belgium; UCLouvain, Université Catholique de Louvain, Louvain Drug Research Institute (LDRI), Metabolism and Nutrition Research Group (MNUT), Brussels, Belgium; Walloon Excellence in Life Sciences and BIOtechnology (WELBIO), WELBIO Department, WEL Research Institute, Wavre, Belgium; UCLouvain, Université Catholique de Louvain, Institute of Experimental and Clinical Research (IREC), 1200 Brussels, Belgium.

出版信息

STAR Protoc. 2024 Dec 20;5(4):103416. doi: 10.1016/j.xpro.2024.103416. Epub 2024 Nov 1.

DOI:10.1016/j.xpro.2024.103416
PMID:39487982
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11565454/
Abstract

Here, we present a protocol for the pre-clinical evaluation of gut barrier function and immune interaction in an HT-29/PBMC co-culture model. We describe steps for culturing HT-29 cells and peripheral blood mononuclear cells (PBMCs), assembling the co-culture model, and testing cell viability. We then detail procedures for performing efficacy tests through stress challenges and barrier permeability assays. For complete details on the use and execution of this protocol, please refer to Abot et al..

摘要

在此，我们展示了一种在HT-29/PBMC共培养模型中对肠道屏障功能和免疫相互作用进行临床前评估的方案。我们描述了培养HT-29细胞和外周血单核细胞（PBMCs）、组装共培养模型以及测试细胞活力的步骤。然后，我们详细说明了通过应激挑战和屏障通透性测定进行功效测试的程序。有关该方案的使用和执行的完整详细信息，请参考阿博特等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/0c20bf8f5798/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/1723243a16f5/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/ca4bb8fe9cfd/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/981f289aae4a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/eb81f522ac7f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/a2b1eb23bb41/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/82ed5b9ff237/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/0c20bf8f5798/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/1723243a16f5/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/ca4bb8fe9cfd/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/981f289aae4a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/eb81f522ac7f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/a2b1eb23bb41/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/82ed5b9ff237/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ed7/11565454/0c20bf8f5798/gr6.jpg

相似文献

1
Protocol for the preclinical evaluation of gut barrier function and immune interaction in an HT-29/PBMC co-culture model.HT-29/PBMC共培养模型中肠道屏障功能和免疫相互作用的临床前评估方案。
STAR Protoc. 2024 Dec 20;5(4):103416. doi: 10.1016/j.xpro.2024.103416. Epub 2024 Nov 1.
2
Protocol for monitoring phagocytosis of cancer cells by TAM-like macrophages using imaging cytometry.使用成像细胞术监测类TAM巨噬细胞对癌细胞吞噬作用的实验方案。
STAR Protoc. 2024 Dec 20;5(4):103320. doi: 10.1016/j.xpro.2024.103320. Epub 2024 Sep 18.
3
Exposure of intestinal epithelial cells to UV-killed Lactobacillus GG but not Bifidobacterium breve enhances the effector immune response in vitro.肠上皮细胞暴露于经紫外线杀死的鼠李糖乳杆菌 GG 但不是短双歧杆菌可增强体外效应免疫应答。
Int Arch Allergy Immunol. 2010;152(2):159-68. doi: 10.1159/000265537. Epub 2009 Dec 16.
4
Human monocyte-derived dendritic cells from leukoreduction system chambers after plateletpheresis are functional in an in vitro co-culture assay with intestinal epithelial cells.从血小板单采后的白细胞减少系统腔室中分离的人单核细胞来源的树突状细胞在与肠上皮细胞的体外共培养测定中具有功能。
J Immunol Methods. 2012 Oct 31;384(1-2):164-70. doi: 10.1016/j.jim.2012.07.005. Epub 2012 Jul 24.
5
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC.一种用于研究X射线照射的Caco-2细胞与外周血单核细胞之间相互作用的共培养方法。
J Vis Exp. 2018 Jan 30(131):56908. doi: 10.3791/56908.
6
Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy.用于模拟肿瘤-免疫相互作用并评估治疗效果的患者来源临床前平台方案。
STAR Protoc. 2025 Mar 21;6(1):103623. doi: 10.1016/j.xpro.2025.103623. Epub 2025 Feb 6.
7
Patients with inflammatory bowel disease (IBD) reveal increased induction capacity of intracellular interferon-gamma (IFN-gamma) in peripheral CD8+ lymphocytes co-cultured with intestinal epithelial cells.炎症性肠病（IBD）患者外周血CD8 +淋巴细胞与肠上皮细胞共培养时，细胞内干扰素-γ（IFN-γ）的诱导能力增强。
Clin Exp Immunol. 2001 Jan;123(1):15-22. doi: 10.1046/j.1365-2249.2001.01443.x.
8
co-culture of human intestinal organoids and lamina propria-derived CD4 T cells.人肠类器官与固有层来源的 CD4 T 细胞的共培养。
STAR Protoc. 2021 May 14;2(2):100519. doi: 10.1016/j.xpro.2021.100519. eCollection 2021 Jun 18.
9
Supernatants of HIV-infected immune cells affect the barrier function of human HT-29/B6 intestinal epithelial cells.感染HIV的免疫细胞的上清液会影响人HT-29/B6肠上皮细胞的屏障功能。
AIDS. 2002 May 3;16(7):983-91. doi: 10.1097/00002030-200205030-00004.
10
IL-10 producing CD14low monocytes inhibit lymphocyte-dependent activation of intestinal epithelial cells by commensal bacteria.
Microbiol Immunol. 2002;46(3):195-205. doi: 10.1111/j.1348-0421.2002.tb02686.x.

本文引用的文献

1
Effect of the dietary supplement PERMEAPROTECT+ TOLERANCE© on gut permeability in a human co-culture epithelial and immune cells model.膳食补充剂PERMEAPROTECT+ TOLERANCE©对人上皮细胞与免疫细胞共培养模型中肠道通透性的影响。
Heliyon. 2024 Mar 27;10(7):e28320. doi: 10.1016/j.heliyon.2024.e28320. eCollection 2024 Apr 15.
2
The Influence of Nutritional Factors on Immunological Outcomes.营养因素对免疫结果的影响。
Front Immunol. 2021 May 31;12:665968. doi: 10.3389/fimmu.2021.665968. eCollection 2021.
3
Intestinal cell culture models and their potential to study the effect of food components on intestinal inflammation.
肠道细胞培养模型及其在研究食物成分对肠道炎症影响中的潜力。
Crit Rev Food Sci Nutr. 2019;59(22):3648-3666. doi: 10.1080/10408398.2018.1506734. Epub 2018 Oct 2.
4
Human monocyte-derived dendritic cells from leukoreduction system chambers after plateletpheresis are functional in an in vitro co-culture assay with intestinal epithelial cells.从血小板单采后的白细胞减少系统腔室中分离的人单核细胞来源的树突状细胞在与肠上皮细胞的体外共培养测定中具有功能。
J Immunol Methods. 2012 Oct 31;384(1-2):164-70. doi: 10.1016/j.jim.2012.07.005. Epub 2012 Jul 24.
5
Apical TLR ligation of intestinal epithelial cells drives a Th1-polarized regulatory or inflammatory type effector response in vitro.肠道上皮细胞顶端 TLR 连接在体外驱动 Th1 极化的调节性或炎症型效应器反应。
Immunobiology. 2011 Apr;216(4):518-27. doi: 10.1016/j.imbio.2010.08.005. Epub 2010 Aug 21.
6
Exposure of intestinal epithelial cells to UV-killed Lactobacillus GG but not Bifidobacterium breve enhances the effector immune response in vitro.肠上皮细胞暴露于经紫外线杀死的鼠李糖乳杆菌 GG 但不是短双歧杆菌可增强体外效应免疫应答。
Int Arch Allergy Immunol. 2010;152(2):159-68. doi: 10.1159/000265537. Epub 2009 Dec 16.
7
The roles of tumor necrosis factor-alpha in colon tight junction protein expression and intestinal mucosa structure in a mouse model of acute liver failure.肿瘤坏死因子-α在急性肝衰竭小鼠模型中对结肠紧密连接蛋白表达及肠黏膜结构的作用
BMC Gastroenterol. 2009 Sep 22;9:70. doi: 10.1186/1471-230X-9-70.
8
Enteral and parenteral nutrition distinctively modulate intestinal permeability and T cell function in vitro.肠内营养和肠外营养在体外对肠道通透性和T细胞功能有显著不同的调节作用。
Eur J Nutr. 2009 Feb;48(1):12-21. doi: 10.1007/s00394-008-0754-3. Epub 2008 Nov 8.