HT-29/PBMC共培养模型中肠道屏障功能和免疫相互作用的临床前评估方案。
Protocol for the preclinical evaluation of gut barrier function and immune interaction in an HT-29/PBMC co-culture model.
作者信息
Astre Gwendoline, Créchet Laurianne, Pomié Nicolas, Pereira Ophélie, Cani Patrice D, Knauf Claude, Abot Anne
机构信息
Enterosys SAS, Labège, France.
NeuroMicrobiota, International Research Program (IRP) INSERM/UCLouvain, France/Belgium; UCLouvain, Université Catholique de Louvain, Louvain Drug Research Institute (LDRI), Metabolism and Nutrition Research Group (MNUT), Brussels, Belgium; Walloon Excellence in Life Sciences and BIOtechnology (WELBIO), WELBIO Department, WEL Research Institute, Wavre, Belgium; UCLouvain, Université Catholique de Louvain, Institute of Experimental and Clinical Research (IREC), 1200 Brussels, Belgium.
出版信息
STAR Protoc. 2024 Dec 20;5(4):103416. doi: 10.1016/j.xpro.2024.103416. Epub 2024 Nov 1.
Here, we present a protocol for the pre-clinical evaluation of gut barrier function and immune interaction in an HT-29/PBMC co-culture model. We describe steps for culturing HT-29 cells and peripheral blood mononuclear cells (PBMCs), assembling the co-culture model, and testing cell viability. We then detail procedures for performing efficacy tests through stress challenges and barrier permeability assays. For complete details on the use and execution of this protocol, please refer to Abot et al..
在此，我们展示了一种在HT-29/PBMC共培养模型中对肠道屏障功能和免疫相互作用进行临床前评估的方案。我们描述了培养HT-29细胞和外周血单核细胞（PBMCs）、组装共培养模型以及测试细胞活力的步骤。然后，我们详细说明了通过应激挑战和屏障通透性测定进行功效测试的程序。有关该方案的使用和执行的完整详细信息，请参考阿博特等人的研究。
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