co-culture of human intestinal organoids and lamina propria-derived CD4 T cells.

Crosstalk between immune cells and intestinal stem cells (ISCs) plays a critical role in tissue homeostasis and inflammation; however, models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous long-term 3D co-culture system of human intestinal CD4 T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019).