Ferte N, Jacquot J P, Meunier J C
Eur J Biochem. 1986 Feb 3;154(3):587-95. doi: 10.1111/j.1432-1033.1986.tb09439.x.
This paper compares structural, immunological and kinetic properties of corn (C4) and spinach (C3) NADP-malate dehydrogenases. These chloroplastic enzymes are regulated in vivo by thiol-disulfide interchange. Both in their oxidized (inactive) and reduced (active) states these enzymes have a dimeric structure with molecular masses for the subunit ranging from 28 kDa to 38 kDa according to the procedure used for the determination. These enzymes are thus structurally related. The use of specific antibodies showed that they are also immunologically related although not identical. Finally both enzymes showed close kinetic properties with comparable kcat and Km. Since C4 plants have approximately ten times more NADP-malate dehydrogenase activity than C3 plants, these data suggest that the differences in activities are probably related to the enzyme content of each plant type.
本文比较了玉米（C4）和菠菜（C3）NADP - 苹果酸脱氢酶的结构、免疫学和动力学特性。这些叶绿体酶在体内通过硫醇 - 二硫键互换进行调节。无论是氧化态（无活性）还是还原态（有活性），根据所使用的测定方法，这些酶均具有二聚体结构，亚基的分子量范围为28 kDa至38 kDa。因此，这些酶在结构上相关。使用特异性抗体表明，它们在免疫学上也相关，尽管并不完全相同。最后，两种酶都表现出相近的动力学特性，kcat和Km相当。由于C4植物的NADP - 苹果酸脱氢酶活性大约是C3植物的十倍，这些数据表明活性差异可能与每种植物类型的酶含量有关。
