Quantitative representation of two germ-line V genes in the early antibody response to 2-phenyloxazolone.

Early anti-phenyloxazolone antibodies of BALB/c mice include a subset that bears the nearly or totally unmutated VH-Ox1 sequence. A fraction of this subset also bears the nearly or totally unmutated V kappa-Ox1 sequence. The whole subset is recognized by an anti-idiotype serum 495 and the VH-Ox1/V kappa-Ox1 fraction also by another anti-idiotype serum 260. Frequencies of the VH-Ox1 subset and its V kappa-Ox1 fraction in early IgM and IgG antibodies were determined by typing hybridomas with anti-idiotype antisera. The whole subset appears to make up approximately one half of IgG antibodies, two thirds of this being 495+/260+ in both isotypes. IgM data are less certain but the same frequencies may be valid. Affinities of 495+/260+ antibodies ranged from 1.3 X 10(6) to 11 X 10(6), affinities of 495+/260- antibodies from 0.47 X 10(6) to 1.1 X 10(6), and affinities of doubly negative antibodies were less than 0.41 X 10(6). High affinity is probably an explanation for the high proportion (one third) of the 495+/260+ antibodies in the early response. Doubly negative (and low-affinity) hybridomas may not have been classified as producers of phenyloxazolone antibodies in earlier studies, and this could explain the still higher reported frequency (73%) of VH-Ox1/V kappa-Ox1 antibodies.