Department of Toxicology and Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Department of Biochemistry and Metabolism, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Toxicon. 2024 Nov 28;251:108160. doi: 10.1016/j.toxicon.2024.108160. Epub 2024 Nov 1.
Ochratoxin A (OTA) is a mycotoxin that causes major health concerns in human and animals. Quercetin (QUE) is a flavonoid that possesses antioxidant, anti-inflammatory and anti-apoptotic properties. This report aims to investigate the ameliorative effects of QUE against OTA-induced hepatotoxicity in broiler chicken. Forty broiler chicks were equally allocated into 4 groups: Group I (control), Group II (OTA), Group III (QUE) and Group IV (OTA + QUE). OTA (0.5 mg/kg) and QUE (0.5 g/kg) were incorporated into the chicken feed for 42 days. The results presented a significant decrease in body weight and elevation in feed conversion ratio, and a significant elevation of the activities of serum alanine aminotransferase and aspartate aminotransferase enzymes in the OTA birds. Additionally, there was a significant decrease in catalase activity and reduced glutathione content and a significant elevation in malondialdehyde level in the liver of OTA-exposed birds. Various hepatocellular lesions were also noticed in the OTA-exposed birds. OTA exposure up-regulated the phosphatase and tensin homologue (PTEN) and the pro-apoptotic genes and down-regulated the anti-apoptotic genes in the liver. The addition of QUE ameliorated most of the hepatotoxic effects of OTA.
赭曲霉毒素 A（OTA）是一种真菌毒素，会对人类和动物的健康造成重大影响。槲皮素（QUE）是一种类黄酮，具有抗氧化、抗炎和抗细胞凋亡的特性。本报告旨在研究 QUE 对肉鸡 OTA 诱导的肝毒性的改善作用。将 40 只肉鸡平均分配到 4 组：第 I 组（对照组）、第 II 组（OTA）、第 III 组（QUE）和第 IV 组（OTA+QUE）。OTA（0.5mg/kg）和 QUE（0.5g/kg）被掺入鸡饲料中 42 天。结果显示，OTA 组的体重显著下降，饲料转化率升高，血清丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性显著升高。此外，OTA 暴露组的肝脏中过氧化氢酶活性降低，谷胱甘肽含量降低，丙二醛水平升高。OTA 暴露组的鸡还出现了各种肝细胞病变。OTA 暴露上调了磷酸酶和张力蛋白同源物（PTEN）和促凋亡基因，下调了抗凋亡基因。添加 QUE 改善了 OTA 的大部分肝毒性作用。
