State Key Laboratory for Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China.
State Key Laboratory for Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China; School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212003, China.
Anal Chim Acta. 2024 Nov 22;1330:343297. doi: 10.1016/j.aca.2024.343297. Epub 2024 Oct 2.
Cereals are susceptible to aflatoxin contamination during storage and transportation, which is highly carcinogenic and teratogenic, and seriously threaten human health. The accurate and rapid detection of total aflatoxin (including aflatoxin B1, B2, G1, and G2) is of great importance for food safety. Conventional fluorescence immunoassays have the advantage of being sensitive and fast; however, these methods can be affected by strong background and matrix interference. Therefore, the development of ultrasensitive, cost-effective, and interference rejection sensors for detecting aflatoxins in moldy grains is vital for food safety and human health.
In this paper, a broad-spectrum aflatoxin monoclonal antibody was prepared by using hybridoma cell fusion technology. An aggregation-induced emission (AIE) based immunosensor via silver amplification coupled with a fluorogenic Ag probe was established for AFB analysis. Silver nanoparticles are decomposed into numerous Ag by HO, and then Ag further specifically binds with imidazole-modified AIE molecules, improving the sensitivity and anti-interference ability of the method. The IC and IC of AIE-based immunosensor for AFB were 0.019 and 0.0014 μg/L, respectively, 2.3-fold and 5.8-fold higher than those of icELISA. The AIE-based immunosensor was also used to analyze AFB from actual cereal samples, with spiked recoveries ranging from 72.91 to 115.92 %. In addition, the method was used to detect total aflatoxins in moldy grains.
Based on the advantages of broad-spectrum aflatoxin monoclonal antibody, high-efficiency metal signal amplification, and functional AIE molecule, a sensitive, accurate, cost-effective, and time-saving method was developed for the analysis of total aflatoxins in cereals. Moreover, the proposed signal amplification strategy shows great potential for analyzing other trace-level small molecular pollutants.
谷物在储存和运输过程中容易受到黄曲霉毒素的污染，黄曲霉毒素具有高度致癌性和致畸性，严重威胁着人类健康。准确、快速地检测总黄曲霉毒素（包括黄曲霉毒素 B1、B2、G1 和 G2）对于食品安全至关重要。传统的荧光免疫分析具有灵敏、快速的优点；然而，这些方法可能会受到强背景和基质干扰的影响。因此，开发用于检测霉变谷物中黄曲霉毒素的超灵敏、经济高效且抗干扰的传感器对于食品安全和人类健康至关重要。
本文采用杂交瘤细胞融合技术制备了一种广谱黄曲霉毒素单克隆抗体。建立了一种基于聚集诱导发射（AIE）的免疫传感器，通过银放大结合荧光 Ag 探针用于 AFB 分析。HO 将纳米银颗粒分解成大量 Ag，然后 Ag 进一步与咪唑修饰的 AIE 分子特异性结合，提高了方法的灵敏度和抗干扰能力。基于 AIE 的免疫传感器检测 AFB 的 IC 和 IC 分别为 0.019 和 0.0014μg/L，分别比 icELISA 高 2.3 倍和 5.8 倍。该基于 AIE 的免疫传感器还用于分析实际谷物样品中的 AFB，加标回收率在 72.91%至 115.92%之间。此外，该方法还用于检测霉变谷物中的总黄曲霉毒素。
基于广谱黄曲霉毒素单克隆抗体、高效金属信号放大和功能 AIE 分子的优势，开发了一种灵敏、准确、经济高效、省时的谷物总黄曲霉毒素分析方法。此外，所提出的信号放大策略在分析其他痕量小分子污染物方面具有很大的潜力。
