高度乙酰化染色质的结构：光散射和流动线性二色性研究

Structure of hyperacetylated chromatin: light scattering and flow linear dichroism study.

作者信息

Dimitrov S, Makarov V, Apostolova T, Pashev I

出版信息

FEBS Lett. 1986 Mar 3;197(1-2):217-20. doi: 10.1016/0014-5793(86)80329-5.

Abstract

Cation-induced folding of 10 nm chromatin filament to 30 nm fiber was studied with hyperacetylated chromatin using light scattering at 90 degrees and flow linear dichroism. Acetylated chromatin folded in a way indistinguishable from that of the control chromatin: both the compactness of chromatin and the orientation of nucleosomes relative to the fiber axis were identical at a given salt concentration.

摘要

利用90度光散射和流动线性二色性，对阳离子诱导的10纳米染色质细丝折叠成30纳米纤维进行了研究，研究对象为高度乙酰化的染色质。乙酰化染色质的折叠方式与对照染色质无法区分：在给定盐浓度下，染色质的紧密程度以及核小体相对于纤维轴的取向均相同。