Zhang Feiyue, Ma Ning, He Lingxiao, Lin Hanchen, Wei Shuangyu, Zhao Xinxu, You Chaoqun, Cai Lingchao, Wang Fei
Jiangsu Co-Innovation Centre of Efficient Processing and Utilization of Forest Resources, Jiangsu Key Lab for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, PR China.
Jiangsu Co-Innovation Centre of Efficient Processing and Utilization of Forest Resources, Jiangsu Key Lab for the Chemistry and Utilization of Agro-Forest Biomass, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, PR China.
Int J Biol Macromol. 2024 Dec;282(Pt 2):136928. doi: 10.1016/j.ijbiomac.2024.136928. Epub 2024 Oct 26.
Covalent organic structures (MOFs), known for their exceptional properties in separation and purification, have garnered significant attention. However, applying MOF-based adsorbents in complex flavonoid separation scenarios remains challenging. In this study, we successfully synthesized adsorbent materials capable of efficiently adsorbing flavonoids using a vacuum freeze-drying method. The materials were derived from a cross-linked chitosan aerogel functionalized with amino-substituted zeolite imidazolium ester skeleton (ZIF-8). The ZIF-8-NH, synthesized via in situ substitution of 2-aminobenzimidazole within the ZIF-8 framework, exhibits a smaller pore size than mono ligand ZIF-8. Due to its synergistic interaction with chitosan's biocompatibility and porous structure, the aerogel material (ZIF-8-NH/CS) exhibited outstanding adsorption capacities (183.37 mg/g, 226.34 mg/g, and 187.16 mg/g) in standard solutions (T = 318 K, rpm = 200) of luteolin, quercetin, and rutin, along with high adsorption rates (71.3 ± 2.3 %, 72.4 ± 1.4 %, and 70.7 ± 3.5 %). And showed rapid adsorption in the first 60 min. After 5 cycles, the adsorption capacity of ZIF-8-NH/CS aerogel remained at 80.5 % of the adsorption capacity of the initial cycle, and therefore, ZIF-8-NH/CS aerogel has a good potential for reusability. Additionally, the adsorption process adhered to pseudo-first-order and pseudo-second-order kinetic models, alongside Langmuir and Freundlich isothermal adsorption models. This study introduces novel ideas and methods for the extraction and separation of flavonoids. Furthermore, the developed ZIF-8-NH/CS aerogels with amino functionality hold promise for diverse applications in separating and purifying bioactive substances.
共价有机结构（MOFs）以其在分离和纯化方面的卓越性能而闻名，已引起广泛关注。然而，将基于MOF的吸附剂应用于复杂的黄酮类化合物分离场景仍然具有挑战性。在本研究中，我们通过真空冷冻干燥法成功合成了能够有效吸附黄酮类化合物的吸附剂材料。这些材料源自用氨基取代的沸石咪唑酯骨架（ZIF-8）功能化的交联壳聚糖气凝胶。通过在ZIF-8骨架内原位取代2-氨基苯并咪唑合成的ZIF-8-NH，其孔径比单配体ZIF-8小。由于其与壳聚糖的生物相容性和多孔结构的协同相互作用，气凝胶材料（ZIF-8-NH/CS）在木犀草素、槲皮素和芦丁的标准溶液（T = 318 K，转速 = 200）中表现出出色的吸附容量（183.37 mg/g、226.34 mg/g和187.16 mg/g），以及高吸附率（71.3 ± 2.3%、72.4 ± 1.4%和70.7 ± 3.5%）。并且在前60分钟内显示出快速吸附。经过5个循环后，ZIF-8-NH/CS气凝胶的吸附容量保持在初始循环吸附容量的80.5%，因此，ZIF-8-NH/CS气凝胶具有良好的可重复使用潜力。此外，吸附过程符合准一级和准二级动力学模型，以及朗缪尔和弗伦德利希等温吸附模型。本研究为黄酮类化合物的提取和分离引入了新的思路和方法。此外，开发的具有氨基功能的ZIF-8-NH/CS气凝胶在生物活性物质的分离和纯化方面具有多种应用前景。
