Guangxi Key Laboratory of High-Quality Formation and Utilization of Dao-di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China.
Gene. 2025 Jan 30;935:149054. doi: 10.1016/j.gene.2024.149054. Epub 2024 Oct 28.
Mango (Mangifera indica L.) is an important tropical fruit, and timely flowering and fruit setting are very important for mango production. The MADS-box gene family is involved in the regulation of flower induction, floral organ specification, and fruit development in plants. The identification and analysis of the MADS-box gene family can lay a foundation for the study of the molecular mechanism of flowering and fruit development in mango. In this study, 119 MiMADS-box genes were identified on the basis of genome and transcriptome data. Phylogenetic analysis revealed that these genes can be divided into two classes. Forty-one type I proteins were further divided into three subfamilies, and seventy-eight type II proteins were further classified into eleven subfamilies. Several pairs of alternative splicing genes were found, especially in the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) subfamily. The MiMADS-box genes were distributed on 18 out of the 20 mango chromosomes. Cis-element analysis revealed many light-, stress-, and hormone-responsive elements in the promoter regions of the mango MiMADS-box genes. Expression pattern analysis revealed that these genes were differentially expressed in multiple tissues in mango. The highly expressed MiMADS77 was subsequently transformed into Arabidopsis, resulting in significant early flowering and abnormal floral organs. Yeast two-hybrid (Y2H) assays revealed that MiMADS77 interacts with several MiMADS-box proteins. In addition, we constructed a preliminary flowering regulatory network of MADS-box genes in mango on the basis of related studies. These results suggest that MiMADS77 genes may be involved in flowering regulation of mango.
芒果（Mangifera indica L.）是一种重要的热带水果，适时开花和结实对芒果生产非常重要。MADS 框基因家族参与植物花诱导、花器官特化和果实发育的调控。MADS 框基因家族的鉴定和分析可以为芒果开花和果实发育的分子机制研究奠定基础。在本研究中，基于基因组和转录组数据，鉴定了 119 个 MiMADS 框基因。系统发育分析表明，这些基因可以分为两类。进一步将 41 种 I 型蛋白分为三个亚家族，将 78 种 II 型蛋白进一步分为 11 个亚家族。发现了几对选择性剪接基因，特别是在 SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1（SOC1）亚家族中。MiMADS 框基因分布在芒果的 20 条染色体中的 18 条上。顺式作用元件分析揭示了芒果 MiMADS 框基因启动子区域中许多光、应激和激素响应元件。表达模式分析表明，这些基因在芒果的多个组织中差异表达。高度表达的 MiMADS77 随后被转化到拟南芥中，导致开花提前和花器官异常。酵母双杂交（Y2H）实验表明，MiMADS77 与几种 MiMADS 框蛋白相互作用。此外，我们还根据相关研究构建了芒果 MADS 框基因初步开花调控网络。这些结果表明，MiMADS77 基因可能参与芒果的开花调控。
