使用开放柱色谱法和反相高效液相色谱法从扁形虫中分离性别诱导物质。
Fractionation of a sex-inducing substance from flatworms using open-column chromatography and reverse-phase high-performance liquid chromatography.
作者信息
Sekii Kiyono, Watanabe Taro, Ito Riku, Yoshikawa Akitoshi, Ichikawa-Seki Madoka, Sakamoto Kimitoshi, Kobayashi Kazuya
机构信息
Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan; Faculty of Business and Commerce, Keio University, Hiyoshi 4-1-1, Yokohama, Kanagawa 223-8521, Japan.
Faculty of Agriculture and Life Science, Hirosaki University, Bunkyo-cho 3, Hirosaki, Aomori 036-8561, Japan.
出版信息
STAR Protoc. 2023 Oct 27;4(4):102625. doi: 10.1016/j.xpro.2023.102625.
A substance that sexualizes planarians, an ancestral group of parasitic flatworms, is widely present in planarians and parasitic flatworms. Here, we present a protocol for extracting and purifying the active fraction with sex-inducing activity. We describe steps for homogenization of flatworms, sample concentration, open-column chromatography, and reverse-phase high-performance liquid chromatography. We then detail a feeding bioassay to confirm sex-inducing activity. The obtained active fraction may positively affect parasitic flatworm sexual maturation and can be tested by adding it into the culture media. For complete details on the use and execution of this protocol, please refer to Sekii et al. (2023)..
一种能使涡虫（一种寄生扁虫的原始类群）发生性转变的物质广泛存在于涡虫和寄生扁虫中。在此，我们展示了一种提取和纯化具有性诱导活性的活性组分的方法。我们描述了扁虫匀浆、样品浓缩、开放柱色谱和反相高效液相色谱的步骤。然后，我们详细介绍了一种用于确认性诱导活性的喂食生物测定法。所获得的活性组分可能对寄生扁虫的性成熟有积极影响，可通过将其添加到培养基中进行测试。有关本方法的使用和实施的完整详细信息，请参考Sekii等人（2023年）的文献。
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