Zhao Yunxia, Li Li, Cai Zhaogen
Department of Pathophysiology, Bengbu Medical University, Bengbu, Anhui 233030, P.R. China.
Department of Pathology, Anqing 116 Hospital, Anqing, Anhui 246000, P.R. China.
Oncol Lett. 2024 Oct 23;29(1):22. doi: 10.3892/ol.2024.14767. eCollection 2025 Jan.
The present study aimed to investigate glucoside xylosyltransferase 2 (GXYLT2) as a potential prognostic marker for gastric cancer (GC). The expression levels of GXYLT2, Notch1, E-cadherin and vimentin in GC and adjacent tissues were detected by Elivision™️ Plus immunohistochemistry. The relationship between GXYLT2, Notch1, E-cadherin and vimentin, and clinicopathological parameters was also analyzed. Univariate and multivariate Cox regression analyses were conducted to evaluate the effect of GXYLT2 on survival. The results revealed that GC tissues exhibited a marked increase in GXYLT2, Notch1 and vimentin, and a marked reduction in E-cadherin. The expression of GXYLT2 was related to the differentiation degree of GC and the pathological tumor-node-metastasis (pTNM) stage; the expression of Notch1 was related to the vascular and nerve infiltration of GC; and E-cadherin and vimentin expression were related to patient age, tumor size, tumor differentiation degree, depth of infiltration, lymph node metastasis and pTNM stage. Positive associations existed between GXYLT2 and Notch1 expression, GXYLT2 and vimentin expression, and Notch1 and vimentin expression. Furthermore, the Kaplan-Meier analysis showed that survival and prognosis were associated with factors such as GXYLT2 protein levels, pTNM stage, tumor dimensions, depth of infiltration and lymph node metastasis. Through Cox regression analysis, GXYLT2 was identified as an independent predictor for GC. In conclusion, GXYLT2 may be related to the pathogenesis of GC, and its abnormal expression could be associated with epithelial-mesenchymal transition. Consequently, GXYLT2 may be considered a promising prognostic marker in the context of GC.
本研究旨在探讨葡萄糖苷木糖基转移酶2（GXYLT2）作为胃癌（GC）潜在预后标志物的可能性。采用Elivision™️ Plus免疫组织化学法检测GC组织及癌旁组织中GXYLT2、Notch1、E-钙黏蛋白和波形蛋白的表达水平。同时分析GXYLT2、Notch1、E-钙黏蛋白和波形蛋白与临床病理参数之间的关系。进行单因素和多因素Cox回归分析以评估GXYLT2对生存的影响。结果显示，GC组织中GXYLT2、Notch1和波形蛋白显著升高，E-钙黏蛋白显著降低。GXYLT2的表达与GC的分化程度及病理肿瘤-淋巴结-转移（pTNM）分期有关；Notch1的表达与GC的血管和神经浸润有关；E-钙黏蛋白和波形蛋白的表达与患者年龄、肿瘤大小、肿瘤分化程度、浸润深度、淋巴结转移及pTNM分期有关。GXYLT2与Notch1表达、GXYLT2与波形蛋白表达以及Notch1与波形蛋白表达之间均呈正相关。此外，Kaplan-Meier分析表明，生存和预后与GXYLT2蛋白水平、pTNM分期、肿瘤大小、浸润深度和淋巴结转移等因素有关。通过Cox回归分析，GXYLT2被确定为GC的独立预测因子。总之，GXYLT2可能与GC的发病机制有关，其异常表达可能与上皮-间质转化有关。因此，在GC的背景下，GXYLT2可被视为一种有前景的预后标志物。
