Azar Bahadori Rosita, Shabani Dina, Arjmandrad Elham, Kazerani Mahsa, Rohani Mina, Ramazani Karim Zohreh, Ali-Kheyl Masoud, Nosratabadi Reza, Pourghadamyari Hossein, Zaemi Mohammad Ali
Department of Molecular Genetics, Parand Branch, Islamic Azad University, Tehran, Iran.
Cellular and Molecular Biology, Department of Biology. Science Faculty, Science and Art University, Yazd, Iran.
Int J Mol Cell Med. 2024;13(3):325-336. doi: 10.22088/IJMCM.BUMS.13.3.325.
Coronary artery diseases (CAD) represent a significant global health concern and are recognized as a primary contributor to mortality on a worldwide scale. Early diagnosis of CAD is one of promising goal to manage this disorder. Recent investigations have highlighted the pivotal involvement of microRNAs (miRNAs) in diverse health conditions, notably CAD. The principal objective of this investigation was to identify appropriate miRNAs that could be employed for the early detection of CAD In the present study, we analyzed dataset of CAD (GSE113079) and 100 differentially expressed mRNAs (DEmRNAs) were detected. The miRNAs that have a significant interaction with DEmRNAs were chosen. By computational prediction method, 5 miRNAs (miR-106b-5p, miR-20a-3p, miR-17-3p, miR-146a-5p, and miR-155-3p) were selected. Finally, we assessed the anticipated expression levels of microRNAs in CAD patients and healthy control groups. Our findings revealed a statistically significant elevation solely in the expression level of miR-106b-5p within the CAD group when compared to the control group (p>0.001). Our study demonstrated an elevation in the expression of miR-106b-5p in individuals diagnosed with CAD. This microRNA may be used as a diagnostic biomarker in patients with CAD. However, further investigations are needed to confirm these results.
冠状动脉疾病（CAD）是全球重大的健康问题，被公认为全球范围内死亡率的主要促成因素。CAD的早期诊断是控制这种疾病的一个有前景的目标。最近的研究突出了微小RNA（miRNA）在多种健康状况中的关键作用，尤其是在CAD中。本研究的主要目的是确定可用于CAD早期检测的合适miRNA。在本研究中，我们分析了CAD数据集（GSE113079），并检测到100个差异表达的mRNA（DEmRNA）。选择了与DEmRNA有显著相互作用的miRNA。通过计算预测方法，筛选出5个miRNA（miR-106b-5p、miR-20a-3p、miR-17-3p、miR-146a-5p和miR-155-3p）。最后，我们评估了CAD患者和健康对照组中miRNA的预期表达水平。我们的研究结果显示，与对照组相比，CAD组中仅miR-106b-5p的表达水平有统计学意义的升高（p>0.001）。我们的研究表明，在被诊断为CAD的个体中，miR-106b-5p的表达升高。这种miRNA可能用作CAD患者的诊断生物标志物。然而，需要进一步研究来证实这些结果。
