Hohan Robert, Vlaicu Ovidiu, Bănică Leontina, Tudor Andreea Ioana, Negru Anca, Paraschiv Simona, Oţelea Dan
MSc, Molecular Diagnostics Laboratory, National Institute for Infectious Diseases "Prof. Dr. Matei Balş", No. 1 Dr. Calistrat Grozovici street, Bucharest, 021105, Romania.
PhD, Molecular Diagnostics Laboratory, National Institute for Infectious Diseases "Prof. Dr. Matei Balş", No. 1 Dr. Calistrat Grozovici street, Bucharest, 021105, Romania.
Germs. 2024 Jun 30;14(2):126-135. doi: 10.18683/germs.2024.1425. eCollection 2024 Jun.
To better understand the factors which influence the spread of monkeypox (mpox) infection, the patients that tested positive for mpox virus by real-time PCR in one of the main infectious diseases centers in Bucharest were analyzed in this study, amounting to one third of the confirmed cases in Romania.
Clinical data and laboratory tests were used to build the patient profiles. In the case of positive mpox results, next-generation sequencing of the viral genome was also performed to better comprehend the epidemiology of the infections and the evolutionary path of this virus.
Among 47 patients with clinical suspicion of infection, 18 cases tested positive for mpox by real-time PCR (RT-PCR). Patients were mainly men who have sex with men (MSM), often coinfected with HIV-1 (half of the cases) and presenting with other sexually transmitted infections (STIs). Phylogenetic analysis was performed on 20 samples (15 patients) and indicated that mpox cases in Romania were the result of multiple importing events followed by local spread. A few sequences from European countries (Germany, Italy, France) and USA were found to be closely related to the Romanian sequences. Intra-host evolution was observed and documented in one patient with HIV-1 infection with uncontrolled viremia, showing slightly different mutation profiles in two body compartments.
This study showed that the mpox cases from Romania presented similar clinical, epidemiological and mutational features with those reported by other European countries.
为了更好地了解影响猴痘（mpox）感染传播的因素，本研究分析了布加勒斯特一家主要传染病中心通过实时聚合酶链反应检测出猴痘病毒呈阳性的患者，这些患者占罗马尼亚确诊病例的三分之一。
利用临床数据和实验室检测来建立患者档案。对于猴痘检测结果呈阳性的情况，还对病毒基因组进行了二代测序，以更好地了解感染的流行病学情况以及该病毒的进化路径。
在47例临床怀疑感染的患者中，18例通过实时聚合酶链反应（RT-PCR）检测出猴痘呈阳性。患者主要是男男性行为者（MSM），常合并感染HIV-1（半数病例），并伴有其他性传播感染（STIs）。对20个样本（15名患者）进行了系统发育分析，结果表明罗马尼亚的猴痘病例是多次输入事件后在当地传播的结果。发现来自欧洲国家（德国、意大利、法国）和美国的一些序列与罗马尼亚的序列密切相关。在一名HIV-1感染且病毒血症未得到控制的患者中观察到并记录了病毒在宿主体内的进化情况，该患者两个身体部位的突变谱略有不同。
本研究表明，罗马尼亚的猴痘病例在临床、流行病学和突变特征方面与其他欧洲国家报告的病例相似。
