Butterfield J H, Weiler D
In Vitro Cell Dev Biol. 1986 Mar;22(3 Pt 1):157-63. doi: 10.1007/BF02623503.
To date, the small size and slow growth of eosinophil colonies in vitro has hampered study of cloned eosinophils. We found enhanced eosinophil colony size and numbers in methylcellulose cultures of bone marrow cells utilizing defined supplemented bovine calf serum (DSBCS) in combination with EL4 conditioned medium (EL4-CM). At days 9, 16 and 23 significantly more eosinophil colonies and more cells/colony were present in cultures incubated with DSBCS/EL4-CM than in cultures incubated with fetal calf serum/EL4-CM. The ability to generate large numbers of eosinophils in vitro should facilitate study of cloned eosinophils.
迄今为止，体外嗜酸性粒细胞集落体积小且生长缓慢，这阻碍了对克隆嗜酸性粒细胞的研究。我们发现，在骨髓细胞的甲基纤维素培养物中，使用特定补充的小牛血清（DSBCS）与EL4条件培养基（EL4-CM）结合，嗜酸性粒细胞集落的大小和数量会增加。在第9、16和23天，与胎牛血清/EL4-CM培养的细胞相比，用DSBCS/EL4-CM培养的细胞中嗜酸性粒细胞集落明显更多，且每个集落中的细胞更多。体外大量生成嗜酸性粒细胞的能力应有助于对克隆嗜酸性粒细胞的研究。
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