Bjornson B H, André-Schwartz J, Desforges J F
Exp Hematol. 1982 Mar;10(3):271-6.
Using an accurate technique for staining and scoring soft agar cultures, this study defines the incidence of circulating CFUEOS in normal donors. With whole mononuclear cells as the source of colony stimulating factor (CSF) and fetal calf serum, 49.6% +/- 3.5 of the colonies were eosinophilic; with human placental conditioned media and fetal calf serum 33.2% +/- 12.9 of the colonies were eosinophilic, with AB serum and whole mononuclear cells in the feeder layer 58.6% +/- 9.1 of the colonies were eosinophilic. The percentage of mixed neutrophil/eosinophil colonies was similar under varying culture conditions suggesting the presence of circulating progenitor cells capable of producing both lines.
通过使用精确的技术对软琼脂培养物进行染色和评分，本研究确定了正常供体中循环CFUEOS的发生率。以全单核细胞作为集落刺激因子（CSF）和胎牛血清的来源时，49.6%±3.5%的集落为嗜酸性；以人胎盘条件培养基和胎牛血清时，33.2%±12.9%的集落为嗜酸性，以AB血清和饲养层中的全单核细胞时，58.6%±9.1%的集落为嗜酸性。在不同培养条件下，混合中性粒细胞/嗜酸性粒细胞集落的百分比相似，表明存在能够产生这两种细胞系的循环祖细胞。
