用于复苏应激和非应激金黄色葡萄球菌的改良MPN培养基的评估
Evaluation of an improved MPN medium for recovery of stressed and nonstressed Staphylococcus aureus.
作者信息
Lancette G A, Peeler J T, Lanier J M
出版信息
J Assoc Off Anal Chem. 1986 Jan-Feb;69(1):44-6.
Two media used for recovery of foodborne Staphylococcus aureus were compared: trypticase soy broth with 10% salt (TSBS), used in the official AOAC method, and TSBS with 1% sodium pyruvate added (PTSB). Adding pyruvate increased recovery of 4 heat-stressed and nonstressed S. aureus foodborne strains from artificially contaminated mashed potato and ham salad substrates. The overall geometric means for the 2 media differed significantly at the alpha = 0.05 level. Recovery of S. aureus from 70 replicates of naturally contaminated Monterey Jack cheese was highest in PTSB. The overall geometric means/g were 63 in TSBS and 96 in PTSB.
对用于分离食源性金黄色葡萄球菌的两种培养基进行了比较
官方AOAC方法中使用的含10%盐的胰蛋白胨大豆肉汤（TSBS），以及添加了1%丙酮酸钠的TSBS（PTSB）。添加丙酮酸钠提高了从人工污染的土豆泥和火腿沙拉基质中分离4株热应激和非应激食源性金黄色葡萄球菌菌株的回收率。在α = 0.05水平下，两种培养基的总体几何平均值差异显著。从70份自然污染的蒙特里杰克干酪重复样本中分离金黄色葡萄球菌，PTSB中的回收率最高。TSBS中的总体几何平均值为每克63个，PTSB中的为每克96个。
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