Roles of and Genes Expression in Cytogenetically Normal Acute Myeloid Leukemia.

BACKGROUND

Acute myeloid leukemia (AML) has a heterogeneous molecular profile, clinical presentations, and response to treatments and outcomes. DNA methylation is conducted by DNA methyltransferases including DNMT3B. Poly ADP-ribose polymerase 1 belongs to a family of enzymes that mediate important cellular processes including DNA repair, transcription, and cell death/cell proliferation, and it is involved in the development, spread, treatment, and prognosis of some cancers. The objective of this study is to assess the impact of and genes expression on laboratory characteristics, response to treatment and survival in Egyptian cytogenetically normal AML patients.

METHODS

This study included 67 Egyptian CN-AML patients in addition to 8 healthy bone marrow donors. Measurement of and gene expression was done on bone marrow samples via real-time semiquantitative polymerase chain reaction.

RESULT

Expression of both and genes was significantly upregulated in AML ( = .001,  = .036, respectively). Upregulated was associated with higher total leukocyte count (TLC), PB, and BM blast cell%. Also, upregulated correlated with higher TLC, PB, and BM blast cell%. High expression of both and correlated with greater frequencies of . High expression, and combined upregulation of both and genes associated significantly with ELN stratification. But no correlation was found with response (CR), overall survival (OS), disease-free survival (DFS), or event-free survival (EFS).

CONCLUSION

Our findings highlight the importance of considering and expression levels as potential prognostic biomarkers for progression and aggressiveness of CN-AML patients in AML. Assessing their expression levels could be an indicator to guide treatment decisions and potentially improve patient outcomes.