Kim Hyoung-Jun, Yoon Hong Man, Lee Youngjeon, Lim Kyung Seob, Seong Jung Bae, Park Sung-Jae, Choi Yongdoo
Division of Technology Convergence, National Cancer Center, 323 Ilsan-ro, Goyang, Gyeonggi-Do, 10408, Republic of Korea.
Center for Gastric Cancer, National Cancer Center, 323 Ilsan-ro, Goyang, Gyeonggi-Do 10408, Republic of Korea.
Biomater Sci. 2024 Dec 3;12(24):6382-6391. doi: 10.1039/d4bm01252f.
Accurate tumor localization is crucial for the success of minimally invasive surgery, as it minimizes the resection of normal tissues surrounding tumors. Traditional methods for marking gastrointestinal (GI) tumors, such as ink tattooing, intraoperative gastroscopy or colonoscopy, and placement of metal clips, have major drawbacks in their application in laparoscopic surgery. Therefore, the development of safe and easy-to-operate marking methods for accurate and real-time detection of GI tumors during laparoscopic surgery remains an ongoing challenge. Here, we propose a new fluorescent metal clip (MicBall800 clip) for noninvasive and precise fluorescence marking of GI tumors. First, we prepared a poly(methyl methacrylate) microball with small, multiple, and separated pores in its internal structure, and near-infrared fluorescence dye (IRDye800CW) and human serum albumin complex were loaded into the pores. This near-infrared-dye-loaded poly(methyl methacrylate) microball (MicBall800) was then coated onto the surface of the metal clips to produce a highly fluorescent MicBall800 clip. Safety and biocompatibility tests of the MicBall800 clip were conducted by the Korea Testing Certification Institute. The MicBall800 clip was evaluated using a porcine model. The MicBall800 clip passed safety and biocompatibility tests. The MicBall800 clip could be easily marked at the target sites without causing any side effects and was detected in real time during the laparoscopic operation. The data obtained from the safety and biocompatibility tests and the animal study indicate that the MicBall800 clip can be an important advancement in minimally invasive and precision surgery for GI cancers.
准确的肿瘤定位对于微创手术的成功至关重要，因为它能最大程度减少肿瘤周围正常组织的切除。传统的胃肠道（GI）肿瘤标记方法，如墨水纹身、术中胃镜或结肠镜检查以及放置金属夹，在腹腔镜手术中的应用存在重大缺陷。因此，开发一种安全且易于操作的标记方法，以在腹腔镜手术期间准确实时检测GI肿瘤，仍然是一个持续存在的挑战。在此，我们提出一种新型荧光金属夹（MicBall800夹），用于对GI肿瘤进行无创且精确的荧光标记。首先，我们制备了一种内部结构具有小而多且相互分离孔隙的聚甲基丙烯酸甲酯微球，并将近红外荧光染料（IRDye800CW）与人血清白蛋白复合物加载到孔隙中。然后将这种负载近红外染料的聚甲基丙烯酸甲酯微球（MicBall800）涂覆在金属夹表面，制成具有高荧光性的MicBall800夹。MicBall800夹的安全性和生物相容性测试由韩国测试认证机构进行。使用猪模型对MicBall800夹进行评估。MicBall800夹通过了安全性和生物相容性测试。MicBall800夹能够在目标部位轻松标记，且不会引起任何副作用，并且在腹腔镜手术期间能够实时检测到。从安全性和生物相容性测试以及动物研究中获得的数据表明，MicBall800夹可能是GI癌症微创和精准手术的一项重要进展。
