Okiro Lilian A, Mulwa Richard M, Oyoo Maurice E, Ojwang Pascal P Okwiri, Otieno Susan A, Gaiero Paola, Pereira Guilherme da Silva, Mendes Thiago
Department of Crop, Horticulture and Soils, Egerton University, 20115, Njoro, Kenya.
Department of Biochemistry and Molecular Biology, Egerton University, 20115, Njoro, Kenya.
Phytopathology. 2025 Mar;115(3):290-298. doi: 10.1094/PHYTO-06-24-0188-R. Epub 2025 Mar 22.
The development of novel improved varieties adapted to unstable environmental conditions is possible through the genetic diversity of breeding materials. Potato is among the most important food crops worldwide; however, there are still significant hindrances to breeding gains attributed to its autotetraploid and highly heterozygous genome. Bacterial wilt caused by the species complex is an important disease affecting potato among many economically important crops worldwide. No cultivated potato genotypes have shown a satisfactory level of resistance to bacterial wilt. Nevertheless, resistance can play a crucial role in effective integrated disease management. To understand the genetic landscape of bacterial wilt resistance in cultivated potato, we evaluated the diversity of 192 accessions from the International Potato Center (CIP) using 9,250 single-nucleotide polymorphisms and their associations with the response to bacterial wilt disease evaluated over two independent trials. Twenty-four accessions showed high resistance throughout both trials. Genetic diversity analysis revealed three major clusters whose subgroups were mostly represented by CIP clones derived from common parents. Genome-wide association analyses identified six major hits: two on chromosome 8 and one on each chromosome 2, 4, 5, and 9. These results facilitate genetic dissection of bacterial wilt resistance and enable marker-assisted breeding in elite genotypes for potato breeding initiatives. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
通过育种材料的遗传多样性，有可能培育出适应不稳定环境条件的新型改良品种。马铃薯是全球最重要的粮食作物之一；然而，由于其同源四倍体和高度杂合的基因组，育种进展仍然存在重大障碍。由该物种复合体引起的青枯病是全球许多经济作物中影响马铃薯的一种重要病害。没有栽培马铃薯基因型对青枯病表现出令人满意的抗性水平。尽管如此，抗性在有效的综合病害管理中可以发挥关键作用。为了了解栽培马铃薯中青枯病抗性的遗传格局，我们使用9250个单核苷酸多态性评估了国际马铃薯中心（CIP）的192份种质的多样性，以及它们与在两项独立试验中评估的对青枯病反应的关联。24份种质在两项试验中均表现出高抗性。遗传多样性分析揭示了三个主要聚类，其子群体大多由来自共同亲本的CIP克隆代表。全基因组关联分析确定了六个主要位点：位于8号染色体上的两个，以及分别位于2号、4号、5号和9号染色体上的一个。这些结果有助于对青枯病抗性进行遗传剖析，并使马铃薯育种计划中的优良基因型能够进行标记辅助育种。[公式：见正文] 版权所有© 2025作者。这是一篇根据知识共享署名-非商业性使用-禁止演绎4.0国际许可协议分发的开放获取文章。
