抗 OSH1 IgG 的纯化和水稻茎尖分生组织的免疫组织化学染色。
Purification of Anti-OSH1 IgG and Immunohistochemical Staining of Rice Shoot Apical Meristem.
机构信息
National Institute of Genetics, Mishima, Shizuoka, Japan.
出版信息
Methods Mol Biol. 2025;2869:61-73. doi: 10.1007/978-1-0716-4204-7_8.
Immunohistochemical staining (IHC) enables the visualization of protein localization within tissues and organs. Here, we describe IHC to detect the ORYZA SATIVA HOMEOBOX1 (OSH1) protein in sections of rice shoot apical meristems (SAMs). First, anti-OSH1 IgG is purified from OSH1 polyclonal rabbit antisera. For subsequent IHC, rice shoot apices are fixed and embedded in paraffin and sections are prepared for IHC. In this IHC procedure, two different labeling methods, fluorescein isothiocyanate and alkaline phosphatase, are utilized for secondary antibody detection.
免疫组织化学染色（IHC）可使组织和器官内的蛋白质定位可视化。在这里，我们描述了 IHC 来检测水稻茎尖分生组织（SAM）切片中的 ORYZA SATIVA HOMEOBOX1（OSH1）蛋白。首先，从 OSH1 多克隆兔抗血清中纯化抗 OSH1 IgG。对于后续的 IHC，将水稻茎尖固定并嵌入石蜡中，并准备 IHC 切片。在这个 IHC 程序中，使用两种不同的标记方法，荧光素异硫氰酸酯和碱性磷酸酶，来检测二级抗体。
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