Ren Qianqian, Wang Lili, Wang Xin, Min Xiaoran, Dai Xiling, Huang Guozheng, Cao Jianguo
College of Life Sciences, Shanghai Normal University, Shanghai, People's Republic of China.
School of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, People's Republic of China.
J Nat Med. 2025 Jan;79(1):204-214. doi: 10.1007/s11418-024-01854-7. Epub 2024 Nov 5.
Inflammation is a vital and normal physiological response; however, excessive inflammation can contribute to the development of various diseases. Artemisia sieversiana, a traditional Chinese medicinal plant, contains a variety of chemical compounds. One such compound, 3-oxo-11αH-germacra-1(10)E,4Z-dien-12,6α-olide, a germacranolide sesquiterpenoid (germacranolide, GMO), has not been thoroughly investigated regarding its potential anti-inflammatory properties. In this study, the anti-inflammatory and antioxidant properties of GMO were investigated for the lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells. It was demonstrated that GMO effectively suppressed the production of inflammatory mediators, decreased the phosphorylation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) in RAW264.7 cells. Additionally, GMO exhibited the capacity to mitigate oxidative damage induced by LPS, as indicated by assessments of reactive oxygen species and mitochondrial membrane potential. In summary, GMO possesses significant anti-inflammatory effects by modulating the NF-κB/MAPK pathway and antioxidant effects by regulating ROS production.
炎症是一种重要且正常的生理反应；然而，过度炎症会促进各种疾病的发展。西伯利亚艾蒿是一种传统中药材，含有多种化合物。其中一种化合物，3-氧代-11αH-吉马烷-1(10)E,4Z-二烯-12,6α-内酯，一种吉马烷型倍半萜内酯（吉马烷型内酯，GMO），其潜在的抗炎特性尚未得到充分研究。在本研究中，研究了GMO对RAW264.7细胞中脂多糖（LPS）诱导的炎症的抗炎和抗氧化特性。结果表明，GMO有效抑制了RAW264.7细胞中炎症介质的产生，降低了核因子κB（NF-κB）和丝裂原活化蛋白激酶（MAPK）的磷酸化水平。此外，通过对活性氧和线粒体膜电位的评估表明，GMO具有减轻LPS诱导的氧化损伤的能力。总之，GMO通过调节NF-κB/MAPK途径具有显著的抗炎作用，并通过调节ROS产生具有抗氧化作用。
