Xu Siying, Li Quansheng, Jin Haojie, Li Aining, Wang Yonglin
State Key Laboratory of Efficient Production of Forest Resources, Beijing Key Laboratory for Forest Pest Control, College of Forestry, Beijing Forestry University, Beijing, 100083 China.
Phytopathology. 2025 Mar;115(3):260-268. doi: 10.1094/PHYTO-05-24-0160-R. Epub 2025 Mar 22.
Poplar Cytospora canker, caused by , is one of the most destructive and widespread poplar diseases worldwide, especially in northern China. However, our current understanding of its pathogenic mechanisms remains limited. Here, we show that trehalose biosynthetic genes, such as trehalose-6-phosphate synthase 1 (), trehalose-6-phosphate phosphatase (), and the regulatory subunit (), play important roles in the development and virulence of . . The targeted deletion mutants showed reduced trehalose synthesis and were defective in hyphal growth and conidiation. Deletion of any of the three genes attenuated virulence in poplar twigs, and stronger poplar defense responses were triggered after inoculated by the mutants. Additionally, the mutants exhibited increased sensitivity to HO and cell wall stressors. Taken together, the findings suggest that trehalose biosynthetic genes contribute to fungal development, stress responses, and full virulence in . .
杨树壳囊孢溃疡病由[病原体名称未给出]引起，是全球最具破坏性且分布广泛的杨树病害之一，在中国北方尤为严重。然而，我们目前对其致病机制的了解仍然有限。在此，我们表明海藻糖生物合成基因，如海藻糖-6-磷酸合酶1（[基因名称未给出]）、海藻糖-6-磷酸磷酸酶（[基因名称未给出]）和调节亚基（[基因名称未给出]），在[病原体名称未给出]的发育和毒力中发挥重要作用。靶向缺失突变体显示出海藻糖合成减少，在菌丝生长和分生孢子形成方面存在缺陷。三个基因中的任何一个缺失都会减弱在杨树嫩枝中的毒力，并且在接种突变体后会引发更强的杨树防御反应。此外，突变体对过氧化氢和细胞壁应激源表现出更高的敏感性。综上所述，这些发现表明海藻糖生物合成基因有助于[病原体名称未给出]的真菌发育、应激反应和完全毒力。
