School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, China.
School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, China; Inner Mongolia Key Laboratory of Environmental Pollution Prevention and Waste Resource Recycle, Hohhot 010021, China.
J Environ Manage. 2024 Dec;371:123171. doi: 10.1016/j.jenvman.2024.123171. Epub 2024 Nov 4.
To address the challenges associated with biological treatment of high-salinity wastewater, a novel salt-tolerant strain, Zobellella endophytica W14, was isolated. This strain exhibited heterotrophic nitrification-aerobic denitrification (HN-AD) capabilities. Strain W14 could grow and remove ammonium in high-salinity environments with salinity levels ranging from 0 to 11% (w/v). At 5% salinity, strain W14 demonstrated high removal efficiencies for nitrite, ammonium, and nitrate (100%, 99.58%, and 98.85%, respectively), when these compounds were provided as the single source of nitrogen. In cases of mixed nitrogen sources, total nitrogen removal efficiency of strain reached 95.22%. The nitrogen balance analysis confirmed the utilization of nitrogen sources by strain W14 through both assimilation and dissimilation. Through the amplification of functional genes involved in nitrogen metabolism (i.e., hao, napA, nirS, and nosZ), the nitrogen metabolism pathway of strain W14 was predicted to be: NH₄⁺ → NH₂OH → NO₂⁻ → NO₃⁻ → NO₂⁻ → NO → N₂O → N₂. The study reveals that the novel W14 strain can efficiently remove total nitrogen from high-salinity wastewater and has significant potential for biological treatment of such wastewater.
为了解决高盐废水生物处理所面临的挑战，我们分离到一株新型耐盐菌株，即丛毛单胞菌（Zobellella endophytica）W14。该菌株具有异养硝化-好氧反硝化（HN-AD）能力。W14 菌株能够在盐度为 0 至 11%（w/v）的高盐环境中生长并去除铵。在 5%盐度下，当这些化合物作为唯一氮源时，W14 菌株对亚硝酸盐、铵和硝酸盐的去除效率分别达到 100%、99.58%和 98.85%。在混合氮源的情况下，W14 菌株的总氮去除效率达到 95.22%。氮平衡分析证实了 W14 菌株通过同化和异化作用利用氮源。通过扩增参与氮代谢的功能基因（hao、napA、nirS 和 nosZ），预测 W14 菌株的氮代谢途径为：NH₄⁺ → NH₂OH → NO₂⁻ → NO₃⁻ → NO₂⁻ → NO → N₂O → N₂。本研究表明，新型 W14 菌株能够有效地从高盐废水中去除总氮，具有生物处理此类废水的巨大潜力。
