一种用于检测大象血浆中布洛芬的高效液相色谱法的开发、验证及应用
Development, validation and application of a high-performance liquid chromatography method for the detection of ibuprofen in elephant plasma.
作者信息
Cox Sherry, Bloom Aaron, Golias Riley, Duncan Madeline, Bergman Joan, Cushing Andrew
机构信息
The University of Tennessee, College of Veterinary Medicine, Department of Biomedical and Diagnostic Sciences, 2407 River Drive, Knoxville, TN 37996, USA.
The University of Tennessee, College of Veterinary Medicine, Department of Small Animal Clinical Sciences, 2407 River Drive, Knoxville, TN 37996, USA.
出版信息
Heliyon. 2024 Oct 9;10(20):e39145. doi: 10.1016/j.heliyon.2024.e39145. eCollection 2024 Oct 30.
A simple and accurate method for the determination of ibuprofen concentrations in elephant plasma was developed and validated using reverse phase high-performance liquid chromatography (HPLC). Utilizing a liquid-liquid extraction with ethyl acetate, methanol, and phosphoric acid, samples were separated on an XBridge C column. The mobile phase consisted of 0.005 M ammonium phosphate and acetonitrile (40:60, v/v). Both ibuprofen and the internal standard flurbiprofen were quantitated using an ultraviolet (UV) detector set at 214 nm. The lower limit of quantification (LLOQ) was 0.05 μg/mL in 100 μL of plasma. The average recovery was over 95 % while the intra-assay variability ranged from 0.8 to 9.9 % and the inter-assay variability ranged from 2.7 to 9.8 %. The method was used successfully in a therapeutic drug monitoring program for elephants and could be used in future pharmacokinetic studies.
采用反相高效液相色谱法（HPLC）建立并验证了一种简单准确的测定大象血浆中布洛芬浓度的方法。利用乙酸乙酯、甲醇和磷酸进行液液萃取，样品在XBridge C柱上分离。流动相由0.005 M磷酸铵和乙腈（40:60，v/v）组成。布洛芬和内标氟比洛芬均使用设置在214 nm的紫外（UV）检测器进行定量。在100 μL血浆中，定量下限（LLOQ）为0.05 μg/mL。平均回收率超过95%，批内变异系数范围为0.8%至9.9%，批间变异系数范围为2.7%至9.8%。该方法已成功应用于大象的治疗药物监测项目，可用于未来的药代动力学研究。
Zotero 插件