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对miR160调控不敏感的生长素响应因子10在……中诱导出无孢子生殖样表型。

Auxin response factor 10 insensitive to miR160regulation induces apospory-like phenotypes in .

作者信息

Pessino Silvina, Cucinotta Mara, Colono Carolina, Costantini Elena, Perrone Davide, Di Marzo Maurizio, Callizaya Terceros Giada, Petrella Rosanna, Mizzotti Chiara, Azzaro Celeste, Podio Maricel, Marconi Gianpiero, Albertini Emidio, Dickinson Hugh, Colombo Lucia, Mendes Marta A

机构信息

Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario (UNR), Rosario 2000, Argentina.

Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy.

出版信息

iScience. 2024 Oct 9;27(11):111115. doi: 10.1016/j.isci.2024.111115. eCollection 2024 Nov 15.

DOI:10.1016/j.isci.2024.111115
PMID:39502290
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11536037/
Abstract

The megaspore mother cell (MMC) arises from somatic cells in the ovule primordium and enters meiosis to generate four megaspores. Only the most chalazal (functional megaspore, FM) survives, undergoing a series of mitoses to form the female gametophyte. We show that this commitment to the sexual germline requires spatial regulation of (). -fusion lines reveal expression to be restricted to cells surrounding the MMC in wild type, but ectopically disseminated throughout the ovule in transgenic mARF10 lines insensitive to miR160, an ARF10 downregulator. Significantly, mARF10 ovules develop multiple FMs with differing ploidies, forming putative supernumerary gametophytes with altered polarity and cell identities - features of aposporous apomixis. Furthermore, we confirm the complexity of ovular expression, being mediated by SEEDSTICK, ARGONAUTE1, and miR160. This work adds to our understanding of molecular switches possibly regulating aposporous apomixis, and may contribute the development of innovative plant breeding strategies.

摘要

大孢子母细胞（MMC）起源于胚珠原基中的体细胞，进入减数分裂以产生四个大孢子。只有最靠近合点端的（功能大孢子，FM）存活下来，经过一系列有丝分裂形成雌配子体。我们发现，对有性生殖系的这种定向需要对（）进行空间调控。-融合系显示，在野生型中，（）的表达仅限于围绕MMC的细胞，但在对miR160（一种ARF10下调因子）不敏感的转基因mARF10系中，其异位分布于整个胚珠。重要的是，mARF10胚珠发育出多个具有不同倍性的FM，形成具有改变的极性和细胞身份的假定额外配子体——无孢子生殖的特征。此外，我们证实了胚珠（）表达的复杂性，它由SEEDSTICK、ARGONAUTE1和miR160介导。这项工作增加了我们对可能调控无孢子生殖的分子开关的理解，并可能有助于创新植物育种策略的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/36fb1d20d570/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/6bfd612e579c/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/62f6a10ee197/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/aa759b2557d3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/3a7a97b6d91b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/d51ae51f5db3/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/e442c455677e/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/36fb1d20d570/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/6bfd612e579c/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/62f6a10ee197/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/aa759b2557d3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/3a7a97b6d91b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/d51ae51f5db3/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/e442c455677e/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe0f/11536037/36fb1d20d570/gr6.jpg

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