Lindsay Juanez T, Wirick Matthew J, Karp Xantha
Department of Biology, Central Michigan University.
Biochemistry, Cell and Molecular Biology Program, Central Michigan University.
MicroPubl Biol. 2024 Oct 22;2024. doi: 10.17912/micropub.biology.001358. eCollection 2024.
In adverse conditions, larvae can enter the alternative L2d stage. If conditions remain poor, L2d larvae can molt into stress-resistant dauer larvae. The FOXO ortholog promotes dauer formation, but mutants can enter dauer with incomplete penetrance in combination with a mutation in /TGFβ. The degree to which larvae enter L2d is unknown. Here we show that many mutants express intermediate levels of the L2d marker, suggesting incomplete entry into L2d. Furthermore, lack of expression early in the second larval stage partially predicts which larvae will bypass dauer formation.
在不利条件下,幼虫可进入替代性的L2d阶段。如果条件仍然恶劣,L2d幼虫可蜕皮成为抗逆性的 dauer 幼虫。FOXO 直系同源基因促进 dauer 形成,但突变体与 /TGFβ 突变结合时,进入 dauer 的 penetrance 不完全。幼虫进入L2d的程度尚不清楚。在这里,我们表明许多突变体表达L2d标记的中间水平,表明进入L2d不完全。此外,在第二幼虫阶段早期缺乏表达部分预测了哪些幼虫将绕过 dauer 形成。