• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

冻干骨诱导性细胞外囊泡的功能：一项机制研究。

Functionality of lyophilized osteoinductive EVs: a mechanistic study.

作者信息

Huang Chun-Chieh, Kang Miya, Debnath Koushik, Leung Kasey, Raghavan Vidhath, Lu Yu, Cooper Lyndon F, Gajendrareddy Praveen, Ravindran Sriram

机构信息

Department of Oral Biology, University of Illinois Chicago, Chicago, IL, United States.

Department of Periodontics, University of Illinois Chicago, Chicago, IL, United States.

出版信息

Front Bioeng Biotechnol. 2024 Oct 22;12:1452428. doi: 10.3389/fbioe.2024.1452428. eCollection 2024.

DOI:10.3389/fbioe.2024.1452428
PMID:39502498
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11534714/
Abstract

INTRODUCTION

Mesenchymal stem cell-derived extracellular vesicles (MSC EVs) hold significant promise for regenerative medicine. Lyophilization of EVs significantly enhances their translational potential. While, lyophilized EVs have been studied from a morphological perspective, the functional stability of these EVs and their cargo following lyophilization need to be mechanistically investigated.

METHODS

In this study, we investigated the functional and mechanistic bioactivity of fresh versus lyophilized MSC EVs, specifically focusing on functionally engineered osteoinductive EVs developed in our laboratory. We utilized dimethyl sulfoxide (DMSO) as a cryoprotectant and conducted pathway-specific and experiments to assess the stability and functionality of the EVs.

RESULTS

Our findings show that using DMSO as a cryoprotectant before lyophilization preserves the functional stability of engineered MSC EVs. experiments demonstrated that the endocytosis, cargo integrity, and pathway-specific activity of lyophilized EVs were maintained when DMSO was used as the cryoprotectant. Additionally, bone regeneration studies revealed that the functionality of cryoprotected lyophilized EVs was comparable to that of freshly isolated EVs.

DISCUSSION

These results provide a foundation for evaluating the functionality of lyophilized EVs and exploring the use of DMSO and other cryoprotectants in EV-based therapies. Understanding the functionality of lyophilized naïve and engineered EVs from a mechanistic perspective may enhance validation approaches for tissue regeneration strategies.

摘要

引言

间充质干细胞衍生的细胞外囊泡（MSC EVs）在再生医学领域具有巨大潜力。细胞外囊泡的冻干显著增强了它们的转化潜力。虽然已经从形态学角度对冻干的细胞外囊泡进行了研究，但这些细胞外囊泡及其冻干后的货物的功能稳定性需要进行机制研究。

方法

在本研究中，我们研究了新鲜与冻干的MSC EVs的功能和机制生物活性，特别关注我们实验室开发的功能工程化骨诱导细胞外囊泡。我们使用二甲基亚砜（DMSO）作为冷冻保护剂，并进行了途径特异性实验以评估细胞外囊泡的稳定性和功能。

结果

我们的研究结果表明，在冻干前使用DMSO作为冷冻保护剂可保持工程化MSC EVs的功能稳定性。实验表明，当使用DMSO作为冷冻保护剂时，冻干细胞外囊泡的内吞作用、货物完整性和途径特异性活性得以维持。此外，骨再生研究表明，经冷冻保护的冻干细胞外囊泡的功能与新鲜分离的细胞外囊泡相当。

讨论

这些结果为评估冻干细胞外囊泡的功能以及探索DMSO和其他冷冻保护剂在基于细胞外囊泡的治疗中的应用提供了基础。从机制角度理解冻干的天然和工程化细胞外囊泡的功能可能会增强组织再生策略的验证方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/f8329c8fe05a/fbioe-12-1452428-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/0602a5b4b13f/fbioe-12-1452428-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/88d8eb48bad1/fbioe-12-1452428-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/d27c3962b042/fbioe-12-1452428-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/37b70eb40c16/fbioe-12-1452428-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/94bcf6a5a2b2/fbioe-12-1452428-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/f8329c8fe05a/fbioe-12-1452428-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/0602a5b4b13f/fbioe-12-1452428-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/88d8eb48bad1/fbioe-12-1452428-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/d27c3962b042/fbioe-12-1452428-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/37b70eb40c16/fbioe-12-1452428-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/94bcf6a5a2b2/fbioe-12-1452428-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0488/11534714/f8329c8fe05a/fbioe-12-1452428-g006.jpg

相似文献

1
Functionality of lyophilized osteoinductive EVs: a mechanistic study.冻干骨诱导性细胞外囊泡的功能：一项机制研究。
Front Bioeng Biotechnol. 2024 Oct 22;12:1452428. doi: 10.3389/fbioe.2024.1452428. eCollection 2024.
2
Functionally engineered extracellular vesicles improve bone regeneration.功能工程化细胞外囊泡可改善骨再生。
Acta Biomater. 2020 Jun;109:182-194. doi: 10.1016/j.actbio.2020.04.017. Epub 2020 Apr 16.
3
3D Encapsulation and tethering of functionally engineered extracellular vesicles to hydrogels.三维包封和功能工程细胞外囊泡到水凝胶的系绳。
Acta Biomater. 2021 May;126:199-210. doi: 10.1016/j.actbio.2021.03.030. Epub 2021 Mar 16.
4
Micro RNA based MSC EV engineering: Targeting the BMP2 cascade for bone repair.基于微小RNA的间充质干细胞外泌体工程：靶向骨形态发生蛋白2级联反应促进骨修复
Front Cell Dev Biol. 2023 Feb 8;11:1127594. doi: 10.3389/fcell.2023.1127594. eCollection 2023.
5
Lyoprotectant Constituents Suited for Lyophilization and Reconstitution of Stem-Cell-Derived Extracellular Vesicles.适用于干细胞衍生细胞外囊泡冻干和复溶的冻干保护剂成分。
Biomater Res. 2024 Feb 2;28:0005. doi: 10.34133/bmr.0005. eCollection 2024.
6
Bone regeneration is mediated by macrophage extracellular vesicles.骨再生由巨噬细胞细胞外囊泡介导。
Bone. 2020 Dec;141:115627. doi: 10.1016/j.bone.2020.115627. Epub 2020 Sep 3.
7
Generation of bioactive MSC-EVs for bone tissue regeneration by tauroursodeoxycholic acid treatment.通过牛磺熊去氧胆酸处理生成用于骨组织再生的生物活性间充质干细胞外泌体。
J Control Release. 2023 Feb;354:45-56. doi: 10.1016/j.jconrel.2022.12.053. Epub 2023 Jan 3.
8
Extracellular vesicle-loaded hydrogels for tissue repair and regeneration.用于组织修复和再生的细胞外囊泡负载水凝胶
Mater Today Bio. 2022 Dec 21;18:100522. doi: 10.1016/j.mtbio.2022.100522. eCollection 2023 Feb.
9
Comparative proteomic profiling of Small Extracellular vesicles derived from iPSCs and tissue specific mesenchymal stem cells.iPSCs 和组织特异性间充质干细胞来源的小细胞外囊泡的比较蛋白质组学分析。
Exp Cell Res. 2022 Nov 15;420(2):113354. doi: 10.1016/j.yexcr.2022.113354. Epub 2022 Sep 17.
10
Mesenchymal Stem Cell Derived Extracellular Vesicles for Tissue Engineering and Regenerative Medicine Applications.间质干细胞衍生的细胞外囊泡在组织工程和再生医学中的应用。
Cells. 2020 Apr 16;9(4):991. doi: 10.3390/cells9040991.

本文引用的文献

1
Immunomodulatory properties of naïve and inflammation-informed dental pulp stem cell derived extracellular vesicles.未成熟和炎症相关牙髓干细胞衍生细胞外囊泡的免疫调节特性。
Front Immunol. 2024 Aug 19;15:1447536. doi: 10.3389/fimmu.2024.1447536. eCollection 2024.
2
Lyoprotectant Constituents Suited for Lyophilization and Reconstitution of Stem-Cell-Derived Extracellular Vesicles.适用于干细胞衍生细胞外囊泡冻干和复溶的冻干保护剂成分。
Biomater Res. 2024 Feb 2;28:0005. doi: 10.34133/bmr.0005. eCollection 2024.
3
Prokaryotic microvesicles Ortholog of eukaryotic extracellular vesicles in biomedical fields.
原核微囊泡 生物医学领域真核细胞外囊泡的同源物。
Cell Commun Signal. 2024 Jan 30;22(1):80. doi: 10.1186/s12964-023-01414-8.
4
Therapeutic potential of small extracellular vesicles derived from mesenchymal stem cells for spinal cord and nerve injury.间充质干细胞来源的小细胞外囊泡对脊髓和神经损伤的治疗潜力
Front Cell Dev Biol. 2023 Mar 22;11:1151357. doi: 10.3389/fcell.2023.1151357. eCollection 2023.
5
Micro RNA based MSC EV engineering: Targeting the BMP2 cascade for bone repair.基于微小RNA的间充质干细胞外泌体工程：靶向骨形态发生蛋白2级联反应促进骨修复
Front Cell Dev Biol. 2023 Feb 8;11:1127594. doi: 10.3389/fcell.2023.1127594. eCollection 2023.
6
MicroRNA-based engineering of mesenchymal stem cell extracellular vesicles for treatment of retinal ischemic disorders: Engineered extracellular vesiclesand retinal ischemia.基于 microRNA 的间充质干细胞细胞外囊泡工程治疗视网膜缺血性疾病：工程化细胞外囊泡与视网膜缺血。
Acta Biomater. 2023 Mar 1;158:782-797. doi: 10.1016/j.actbio.2023.01.014. Epub 2023 Jan 11.
7
Mesenchymal stem cell-derived extracellular vesicles for immunomodulation and regeneration: a next generation therapeutic tool?间充质干细胞衍生的细胞外囊泡在免疫调节和再生中的作用：下一代治疗工具？
Cell Death Dis. 2022 Jul 4;13(7):580. doi: 10.1038/s41419-022-05034-x.
8
Extracellular Vesicles From TNFα Preconditioned MSCs: Effects on Immunomodulation and Bone Regeneration.肿瘤坏死因子α预处理间充质干细胞来源的细胞外囊泡：对免疫调节和骨再生的影响。
Front Immunol. 2022 May 2;13:878194. doi: 10.3389/fimmu.2022.878194. eCollection 2022.
9
Emerging Therapeutic Potential of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Chronic Respiratory Diseases: An Overview of Recent Progress.间充质干细胞衍生的细胞外囊泡在慢性呼吸道疾病中的新兴治疗潜力：近期进展概述
Front Bioeng Biotechnol. 2022 Feb 25;10:845042. doi: 10.3389/fbioe.2022.845042. eCollection 2022.
10
Therapeutic roles of mesenchymal stem cell-derived extracellular vesicles in cancer.间充质干细胞衍生的细胞外囊泡在癌症中的治疗作用。
J Hematol Oncol. 2021 Sep 3;14(1):136. doi: 10.1186/s13045-021-01141-y.