Yagita H, Masuko T, Takahashi N, Hashimoto Y
J Immunol. 1986 Mar 15;136(6):2055-61.
It has been shown previously that HBJ127 and HBJ98 monoclonal antibodies raised against a human bladder cancer cell line, and B3 monoclonal antibody against a rat bladder cancer cell line recognized unique cell surface antigens abundant in proliferating cells of the corresponding species. Distribution of the antigens and kinetics of the appearance on human and rat lymphoid cells were examined by means of flow cytometry. Rat macrophages and human peripheral blood monocytes were stained strongly with the B3 and HBJ127 monoclonal antibodies, respectively. With regard to lymphocytes, the expression of the B3-defined antigen on rat lymphocytes was found to have a negative correlation with the maturation of the lymphocytes; the antigen was most abundant in bone marrow cells, less abundant in thymocytes, and least abundant in spleen, lymph node, and peripheral blood lymphocytes. Similarly, the HBJ127-defined antigen on human peripheral lymphocytes was negligible. On activation with Con A or alloantigens, however, both rat and human T lymphocytes did strongly express these antigens. Activation of human or rat B cells with lipopolysaccharide also resulted in the augmented expression of these antigens. Kinetics studies revealed that the antigen expression was readily manifested within 12 hr on activation of rat or human T cells with Con A, was augmented progressively with culture time, and reached a plateau within 36 hr. This somewhat earlier appearance of these antigens apparently preceded the manifestations of the IL 2 receptor (Tac antigen) and the augmented DNA synthesis. The B3-defined antigen on Con A-stimulated T cells was more rich on the lymphocytes in S and G2/M phases than those in G1 phase, and the expression was not significantly affected by the addition of hydroxyurea, but was moderately inhibited by the addition of sodium butylate. These results suggest that the appearance and expression of the B3-defined antigen and probably also those of the HBJ127/HBJ98-defined antigen are correlated with lymphocyte activation and subsequent progression through the cell cycle.
先前的研究表明，针对人膀胱癌细胞系产生的HBJ127和HBJ98单克隆抗体，以及针对大鼠膀胱癌细胞系的B3单克隆抗体，识别在相应物种的增殖细胞中丰富的独特细胞表面抗原。通过流式细胞术检测了这些抗原在人和大鼠淋巴细胞上的分布以及出现的动力学。大鼠巨噬细胞和人外周血单核细胞分别被B3和HBJ127单克隆抗体强烈染色。关于淋巴细胞，发现大鼠淋巴细胞上B3定义的抗原表达与淋巴细胞的成熟呈负相关；该抗原在骨髓细胞中最丰富，在胸腺细胞中较少，在脾脏、淋巴结和外周血淋巴细胞中最少。同样，人外周淋巴细胞上HBJ127定义的抗原可以忽略不计。然而，用刀豆蛋白A或同种异体抗原激活后，大鼠和人T淋巴细胞都强烈表达这些抗原。用脂多糖激活人或大鼠B细胞也导致这些抗原的表达增加。动力学研究表明，在用刀豆蛋白A激活大鼠或人T细胞后12小时内抗原表达很容易显现，随着培养时间逐渐增加，并在36小时内达到平台期。这些抗原的这种稍早出现显然先于IL-2受体（Tac抗原）的表现和DNA合成的增加。刀豆蛋白A刺激的T细胞上B3定义的抗原在S期和G2/M期的淋巴细胞上比在G1期的淋巴细胞上更丰富，并且添加羟基脲对表达没有显著影响，但添加丁酸钠会适度抑制表达。这些结果表明，B3定义的抗原以及可能还有HBJ127/HBJ98定义的抗原的出现和表达与淋巴细胞激活以及随后通过细胞周期的进程相关。
