De Smet W, Vaeck M, Smet E, Brys L, Hamers R
Eur J Immunol. 1983 Nov;13(11):919-28. doi: 10.1002/eji.1830131111.
Several monoclonal antibodies (mAb) against rabbit leukocytes were characterized in binding and functional studies. mAb 1.24 stains thymocytes, bone marrow cells, peripheral T and B cells and blood monocytes. T cells express more 1.24 antigen than B cells. In the absence of added complement (C), mAb 1.24 inhibits alloantigen-, concanavalin A (Con A)-, and phytohemagglutinin (PHA)-, but not pokeweed mitogen (PWM)- or anti-immunoglobulin (Ig)-induced cell proliferation. It also strongly blocks anti-sheep erythrocyte plaque-forming cell responses. A second mAb, designated 4.B9, binds to 20% of thymocytes and to most, if not all, peripheral T cells and in vitro-activated T cell blasts. A third one, 10.B3, is reactive with the nearly entire thymocyte and a major peripheral T cell population. Two-color membrane immunofluorescence reveals the presence of a small population of peripheral blood leukocytes which bear surface Ig and are weakly stained by mAb 4.B9 and 10.B3. Without C, both 4.B9 and 10.B3 inhibit Con A- and PHA-induced mitogenesis, but have no effect on PWM-, antigen-, or alloantigen-induced cell proliferation. Depletion of 4.B9+ cells by panning or complement lysis completely abrogates proliferative responsiveness to antigen and alloantigen, significantly reduces responsiveness to the T cell mitogens Con A and PHA, but enhances that to the B cell mitogen anti-Ig. A fourth mAb, 12.C7, binds to 60% of thymocytes and to 10-30% of peripheral T lymphocytes at high-level fluorescence. T cell blasts obtained in mixed leukocyte reactions are partially stained by mAb 12.C7, while those obtained after Con A or PHA activation are not. In addition, mAb 12.C7 is completely unreactive with B cells or monocytes. Without complement, it does not seem to interfere with any of the in vitro functions tested. All antigens studied here do not appear to be expressed in nonleukon tissues, as they do not bind to erythrocytes and are absent from brain, heart, liver and kidney as shown by quantitative absorption analysis.
通过结合和功能研究对几种抗兔白细胞单克隆抗体（mAb）进行了表征。单克隆抗体1.24可对胸腺细胞、骨髓细胞、外周T细胞和B细胞以及血液单核细胞进行染色。T细胞表达的1.24抗原比B细胞更多。在不添加补体（C）的情况下，单克隆抗体1.24可抑制同种异体抗原、刀豆球蛋白A（Con A）、植物血凝素（PHA）诱导的细胞增殖，但不能抑制美洲商陆有丝分裂原（PWM）或抗免疫球蛋白（Ig）诱导的细胞增殖。它还能强烈阻断抗绵羊红细胞空斑形成细胞反应。第二种单克隆抗体，命名为4.B9，可与20%的胸腺细胞以及大多数（如果不是全部）外周T细胞和体外活化的T细胞母细胞结合。第三种，10.B3，可与几乎全部胸腺细胞和主要的外周T细胞群体发生反应。双色膜免疫荧光显示存在一小部分外周血白细胞，它们带有表面Ig，并且被单克隆抗体4.B9和10.B3弱染色。在没有补体的情况下，4.B9和10.B3均可抑制Con A和PHA诱导的有丝分裂，但对PWM、抗原或同种异体抗原诱导的细胞增殖没有影响。通过淘选或补体裂解去除4.B9 +细胞可完全消除对抗原和同种异体抗原的增殖反应性，显著降低对T细胞有丝分裂原Con A和PHA的反应性，但增强对B细胞有丝分裂原抗Ig的反应性。第四种单克隆抗体，12.C7，可与60%的胸腺细胞以及10 - 30%的外周T淋巴细胞以高水平荧光结合。在混合白细胞反应中获得的T细胞母细胞被单克隆抗体12.C7部分染色，而在Con A或PHA激活后获得的T细胞母细胞则未被染色。此外，单克隆抗体12.C7与B细胞或单核细胞完全无反应。在没有补体的情况下，它似乎不会干扰所测试的任何体外功能。此处研究的所有抗原似乎都不在非白细胞组织中表达，因为它们不与红细胞结合，并且通过定量吸收分析表明在脑、心脏、肝脏和肾脏中不存在。
