Spinner S, Stöffler G, Fink E
J Immunol Methods. 1986 Feb 27;87(1):79-83. doi: 10.1016/0022-1759(86)90346-7.
A competitive ELISA assay has been developed that permits reproducible quantitation of the anticoagulant hirudin in buffer and urine. Coupling of peroxidase and hirudin was performed with the heterobifunctional reagent N-succinimidyl-3-(2-pyridyldithio)-propionate. In both solvents the lower limit of sensitivity was 8 ng hirudin/ml (0.08 AT-U) while the upper limit was 7.7 micrograms/ml (78.45 AT-U).
已开发出一种竞争性酶联免疫吸附测定法，可对缓冲液和尿液中的抗凝剂水蛭素进行可重复定量。使用异双功能试剂N-琥珀酰亚胺基-3-（2-吡啶基二硫代）-丙酸酯将过氧化物酶与水蛭素偶联。在两种溶剂中，灵敏度下限均为8 ng水蛭素/毫升（0.08 AT-U），而上限为7.7微克/毫升（78.45 AT-U）。
