Mykhailova Olga, Brandon-Coatham Mackenzie, Hemmatibardehshahi Sanaz, Yazdanbakhsh Mahsa, Olafson Carly, Yi Qi-Long, Kanias Tamir, Acker Jason P
Innovation and Portfolio Management, Canadian Blood Services, Edmonton, AB, Canada.
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
Blood Adv. 2025 Feb 25;9(4):673-686. doi: 10.1182/bloodadvances.2024014475.
The quality of stored red cell concentrates (RCCs) has been linked to the biological age distribution of red blood cell (RBC) subpopulations. Teenage male RCCs contain higher proportions of biologically old RBCs, with poorer quality. This study sought to assess the contribution of donor sex and age on the deformability characteristics of RBC subpopulations in stored RCCs. On days 5, 14, 28, and 42 of hypothermic storage, RCCs from healthy teenage male (n = 15), senior male (n = 15), teenage female (n = 15), and senior female (n = 15) donors were biologically age profiled. The deformability of the resulting young RBCs and old RBCs (O-RBCs) was assessed using ektacytometry. Over storage, donor age was the biggest factor influencing the rheology of RBC subpopulations. Teenage male RCCs had the largest reduction in Ohyper (osmolality in the hypertonic region corresponding to 50% of the maximum RBC elongation [EImax]). The strongest correlations between Ohyper and mean corpuscular hemoglobin content (R2 > 0.5) were witnessed with O-RBCs from senior donors, and to a lesser extent with teenage males. Teen O-RBCs, particularly from males, had higher elongation indices, both under isotonic conditions and in the presence of an increasing osmotic gradient. Teen RBCs, regardless of biological age, were discovered to be more rigid (higher shear stress required to reach half the EImax). Donor variation in the age distribution of RBC subpopulations and its downstream effect on deformability serves as further evidence that factors beyond storage could potentially affect RCC quality and transfusion outcomes.
储存的红细胞浓缩物（RCCs）的质量与红细胞（RBC）亚群的生物学年龄分布有关。青少年男性的RCCs中生物学上较老的RBC比例较高，质量较差。本研究旨在评估供体性别和年龄对储存的RCCs中RBC亚群变形性特征的影响。在低温储存的第5、14、28和42天，对来自健康青少年男性（n = 15）、老年男性（n = 15）、青少年女性（n = 15）和老年女性（n = 15）供体的RCCs进行生物学年龄分析。使用激光衍射血细胞分析仪评估所得年轻RBC和衰老RBC（O-RBCs）的变形性。在储存过程中，供体年龄是影响RBC亚群流变学的最大因素。青少年男性的RCCs在高渗区渗透压（对应于最大RBC伸长率[EImax]的50%时的渗透压）下降最大。在老年供体的O-RBCs中，观察到Ohyper与平均红细胞血红蛋白含量之间的最强相关性（R2>0.5），在青少年男性中相关性稍弱。青少年O-RBCs，尤其是男性的，在等渗条件下和存在不断增加的渗透梯度时，具有更高的伸长指数。发现青少年RBCs，无论生物学年龄如何，都更僵硬（达到EImax一半所需的剪切应力更高）。RBC亚群年龄分布的供体差异及其对变形性的下游影响进一步证明，储存以外的因素可能会影响RCC质量和输血结果。
