Islamzada Emel, Matthews Kerryn, Lamoureux Erik S, Duffy Simon P, Scott Mark D, Ma Hongshen
Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada.
Centre for Blood Research University of British Columbia Vancouver British Columbia Canada.
EJHaem. 2021 Nov 24;3(1):63-71. doi: 10.1002/jha2.343. eCollection 2022 Feb.
Red blood cells (RBCs) stored in blood bags develop a storage lesion that include structural, metabolic, and morphologic transformations resulting in a progressive loss of RBC deformability. The speed of RBC deformability loss is donor-dependent, which if properly characterized, could be used as a biomarker to select high-quality RBC units for sensitive recipients or to provide customized storage timelines depending on the donor. We used the microfluidic ratchet device to measure the deformability of red blood cells stored in blood bags every 14 days over a span of 56 days. We observed that storage in blood bags generally prevented RBC deformability loss over the current standard 42-day storage window. However, between 42 and 56 days, the deformability loss profile varied dramatically between donors. In particular, we observed accelerated RBC deformability loss for a majority of male donors, but for none of the female donors. Together, our results suggest that RBC deformability loss could be used to screen for donors who can provide stable RBCs for sensitive transfusion recipients or to identify donors capable of providing RBCs that could be stored for longer than the current 42-day expiration window.
储存在血袋中的红细胞会出现一种储存损伤，其中包括结构、代谢和形态学转变，从而导致红细胞变形能力逐渐丧失。红细胞变形能力丧失的速度因供血者而异，如果能够恰当地加以描述，这一指标可作为生物标志物，为敏感受血者筛选高质量的红细胞单位，或者根据供血者情况提供定制化的储存期限。我们使用微流控棘轮装置，在56天的时间跨度内，每14天测量一次储存在血袋中的红细胞的变形能力。我们观察到，在当前标准的42天储存期内，储存在血袋中通常可防止红细胞变形能力丧失。然而，在42天至56天之间，不同供血者的变形能力丧失情况差异巨大。特别是，我们观察到大多数男性供血者的红细胞变形能力加速丧失，但女性供血者均未出现这种情况。我们的研究结果共同表明，红细胞变形能力丧失可用于筛选能够为敏感输血受血者提供稳定红细胞的供血者，或者识别能够提供可储存超过当前42天有效期的红细胞的供血者。
