Ramos Rafael A N, Hakimi Hassan, Metinou Sidouin K, Danzabe Wachellet, Overcast Macon, Cox Jeremiah, Garabed Rebecca, Ouakou Philip Tchindebet, Nare Richard Ngandolo Bongo, Torres-Velez Fernando, Tritten Lucienne, Saleh Meriam N, Verocai Guilherme G
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA; Laboratory of Parasitology, Federal University of the Agreste of Pernambuco, Garanhuns, PE 55282-278, Brazil.
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
Acta Trop. 2024 Dec;260:107454. doi: 10.1016/j.actatropica.2024.107454. Epub 2024 Nov 5.
Cutaneous myiasis caused by various Calliphoridae dipteran species is prevalent worldwide and is of particular veterinary and public health concern. Recently, in a scientific exploration of the Guinea Worm Eradication Program to Chad, Africa, we observed that dogs with mutilated ears, based on local awareness, were caused by cutaneous myiasis. In this study, we analyzed epidemiological, morphological, and molecular data on cutaneous myiasis in dogs from Chad. From September to October 2022, dogs (n = 1,562) from 56 villages situated along the Chari River were physically inspected for cutaneous myiasis. All larvae were collected and identified morphologically and by molecular analysis of the partial cytochrome oxidase c subunit I (COI) mitochondrial gene. The prevalence of myiasis infestation along with 95 % confidence intervals (95 % CI) was determined using the modified Wilson method. Myiasis was detected in dogs from 21 villages (37.5 %; 95 % CI 26 - 50 %), predominating in the southernmost region. Of 1,562 dogs, 66 (4.22 %; 95 % CI 3.34 - 5.34 %) were infested by calliphorid larvae, with a mean infestation of 2.28 larvae per animal (range = 1 to 24). Specimens were morphologically identified as Cordylobia anthropophaga (n = 94), Chrysomya bezziana (n = 54), and Chrysomya sp. (n = 3), which were detected in 57, eight and one dog, respectively. No co-infestations were observed. The molecular analyses confirmed the morphological identification and revealed the presence of 17 haplotypes for C. anthropophaga, 2 for C. bezziana, and one for Chrysomya sp. Our study emphasizes the veterinary importance of myiasis in dogs in Africa and proposes measures to assure their health and well-being.
由各种丽蝇科双翅目物种引起的皮肤蝇蛆病在全球范围内普遍存在，尤其引起兽医和公共卫生关注。最近，在对非洲乍得的几内亚蠕虫根除计划进行的科学探索中，我们观察到，根据当地的认知，耳朵残缺的狗是由皮肤蝇蛆病引起的。在本研究中，我们分析了乍得犬皮肤蝇蛆病的流行病学、形态学和分子数据。2022年9月至10月，对位于沙里河沿岸56个村庄的1562只狗进行了皮肤蝇蛆病的身体检查。收集所有幼虫，并通过形态学和对细胞色素氧化酶c亚基I（COI）线粒体基因部分序列的分子分析进行鉴定。使用改良的威尔逊方法确定蝇蛆病感染率及其95%置信区间（95%CI）。在21个村庄的狗中检测到蝇蛆病（37.5%；95%CI 26 - 50%），最南端地区最为常见。在1562只狗中，66只（4.22%；95%CI 3.34 - 5.34%）被丽蝇幼虫感染，每只动物平均感染2.28只幼虫（范围为1至24只）。标本经形态学鉴定为嗜人瘤蝇（n = 94）、贝氏金蝇（n = 54）和金蝇属（n = 3），分别在57只、8只和1只狗中检测到。未观察到混合感染。分子分析证实了形态学鉴定结果，并揭示嗜人瘤蝇有17个单倍型，贝氏金蝇有2个单倍型，金蝇属有1个单倍型。我们的研究强调了非洲犬皮肤蝇蛆病的兽医重要性，并提出了确保其健康和福祉的措施。
