Knavs Naomi, Ghinet Alina, Lipka Emmanuelle
Univ. Lille, Inserm, Lille, France.
UFR Pharmacie, Laboratoire de Chimie Analytique, Lille, France.
Biomed Chromatogr. 2025 Jan;39(1):e6030. doi: 10.1002/bmc.6030. Epub 2024 Nov 6.
Preparative chromatographic enantioseparation is now the preferred technique for obtaining milligram quantities of pure enantiomers in the initial phase of development of a therapeutic compound. Supercritical fluid chromatography offers several advantages over liquid chromatography and was therefore selected for the preparative enantioseparation of a new potential anti-inflammatory molecule. Approximately 10 mg of each of the two enantiomers was successfully prepared using a Chiralpak AD-H (tris-3,5-dimethylphenylcarbamate of amylose) polysaccharide-based stationary phase with 40% of ethanol as a co-solvent, using a stacked injection mode. A peak distortion was observed during volume overloading, which may be due to the mixed-stream injection method.
制备型色谱对映体分离是目前在治疗性化合物研发初期获取毫克级纯对映体的首选技术。超临界流体色谱相对于液相色谱具有若干优势，因此被选用于一种新型潜在抗炎分子的制备型对映体分离。使用基于直链淀粉的手性固定相Chiralpak AD-H（直链淀粉的三-3,5-二甲基苯基氨基甲酸酯），以40%乙醇作为共溶剂，采用叠加进样模式，成功制备了约10毫克的两种对映体。在进样量过载时观察到峰形畸变，这可能是由于混合流进样方法所致。
