Habermann E, Albus U
J Neurochem. 1986 Apr;46(4):1219-26. doi: 10.1111/j.1471-4159.1986.tb00641.x.
125I-Tetanus toxin is bound by basolateral membranes from rabbit kidneys. Fixation is specific, as it is minimally inhibited by the nonbinding (fragment B) moiety of tetanus toxin, whereas the binding moiety (fragment C) is equivalent to the native toxin in inhibiting fixation. Competition is also pronounced with mildly toxoided toxin. Association and dissociation of 125I-toxin are delayed in kidney when compared to brain membranes. The binding sites in kidney membranes are partially sensitive to neuraminidase and resist heating to 56 degrees C, in contrast to those in brain membranes which are very sensitive to both treatments. The binding sites of the two preparations can be discriminated further by variation of the ionic environment. Sodium dodecyl sulfate-disc gel electrophoresis followed by transfer to nitrocellulose, and TLC with consecutive overlay indicate that tetanus toxin exclusively binds to long-chain gangliosides from rat brain. Binding sites in kidney membranes from rabbits and rats can be made visible by the overlay technique. They are apparently heterogeneous and more hydrophobic. We conclude that rabbit kidney contains binding sites for tetanus toxin which resemble gangliosides but differ from the major gangliosides in brain both chemically and with respect to their interaction with tetanus toxin.
125I-破伤风毒素与兔肾的基底外侧膜结合。这种结合具有特异性，因为破伤风毒素的非结合部分（B片段）对其抑制作用极小，而结合部分（C片段）在抑制结合方面与天然毒素相当。与轻度类毒素化的毒素也存在明显的竞争。与脑膜相比，125I-毒素在肾中的结合和解离过程有所延迟。肾膜中的结合位点对神经氨酸酶部分敏感，并且能耐受加热至56摄氏度，而脑膜中的结合位点对这两种处理都非常敏感。通过改变离子环境可以进一步区分这两种制剂的结合位点。十二烷基硫酸钠-圆盘凝胶电泳后转移至硝酸纤维素膜，以及连续覆盖的薄层层析表明，破伤风毒素仅与大鼠脑内的长链神经节苷脂结合。兔和大鼠肾膜中的结合位点可通过覆盖技术显现出来。它们显然具有异质性且更具疏水性。我们得出结论，兔肾含有破伤风毒素的结合位点，这些位点类似于神经节苷脂，但在化学性质以及与破伤风毒素的相互作用方面与脑中的主要神经节苷脂不同。
