Supple Megan A, Escalona Merly, Alexandre Nicolas, Buchalski Michael R, Riley Seth P D, Dellinger Justin A, Vickers T Winston, Sahasrabudhe Ruta, Nguyen Oanh, Fairbairn Colin W, Seligmann William E, Wilmers Christopher C, Shapiro Beth
Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, CA, United States.
Howard Hughes Medical Institute, University of California, Santa Cruz, CA, United States.
J Hered. 2025 Jul 21;116(4):479-487. doi: 10.1093/jhered/esae063.
Mountain lions, Puma concolor, are widespread and adaptable carnivores. However, due to their large home ranges and long distance dispersals, they are strongly impacted by habitat fragmentation, which results in small and isolated populations. Genomic analyses play an important role in understanding and predicting the impacts of increased isolation of populations, such as decreased genetic diversity and increased levels of inbreeding. Here we report a high-quality, chromosome-level reference genome of P. concolor that was generated as part of the California Conservation Genomics Project. The primary assembly has a total length of 2.5 Gb contained in 258 scaffolds, a contig N50 of 42.3 Mb, a scaffold N50 of 149.8 Mb, and a BUSCO completeness score of 95%. This P. concolor genome assembly will provide an important resource for genomic analyses that aid decision-makers in managing the species in fragmented landscapes.
美洲狮（Puma concolor）是分布广泛且适应性强的食肉动物。然而，由于其活动范围大且扩散距离长，它们受到栖息地破碎化的严重影响，导致种群规模变小且相互隔离。基因组分析在理解和预测种群隔离加剧的影响方面发挥着重要作用，比如遗传多样性降低和近亲繁殖水平增加。在此，我们报告了一个高质量的、染色体水平的美洲狮参考基因组，它是加利福尼亚保护基因组计划的一部分。主要组装体全长2.5 Gb，包含在258个支架中，重叠群N50为42.3 Mb，支架N50为149.8 Mb，BUSCO完整性评分为95%。这个美洲狮基因组组装体将为基因组分析提供重要资源，有助于决策者在破碎化景观中管理该物种。
