猫视网膜中甘氨酸能抑制通路的分析。
Analysis of a glycinergic inhibitory pathway in the cat retina.
作者信息
Wässle H, Schäfer-Trenkler I, Voigt T
出版信息
J Neurosci. 1986 Feb;6(2):594-604. doi: 10.1523/JNEUROSCI.06-02-00594.1986.
Incubation of cat retinas with 3H-glycine in vitro, followed by horizontal sectioning and autoradiography, showed labeling of 10-12% of bipolar cells and 45% of amacrine cells. To ascertain the effects of glycine-accumulating bipolar and amacrine cells on the response properties of retinal ganglion cells, in vivo iontophoretic studies were performed in the cat eye. Glycine inhibited all ganglion cells, and this action was blocked by strychnine. Aminophosphonobutyric acid (APB) suppressed ON-ganglion cells, but activated OFF-ganglion cells. The influence of APB upon OFF-ganglion cells could be completely blocked by strychnine. In the mudpuppy, APB suppressed ON-bipolar cells without affecting OFF-bipolar cells and without direct effects on other retinal neurons (Slaughter and Miller, 1981, 1985). If APB has the same mode of action in the cat retina, the present results suggest a glycinergic inhibitory input of the ON-channel upon OFF-ganglion cells.
将猫视网膜在体外与³H - 甘氨酸一起孵育，随后进行水平切片和放射自显影，结果显示10 - 12%的双极细胞和45%的无长突细胞有标记。为了确定积累甘氨酸的双极细胞和无长突细胞对视网膜神经节细胞反应特性的影响，在猫眼中进行了体内离子电泳研究。甘氨酸抑制所有神经节细胞，且这种作用被士的宁阻断。氨磷丁酸（APB）抑制ON - 神经节细胞，但激活OFF - 神经节细胞。APB对OFF - 神经节细胞的影响可被士的宁完全阻断。在泥螈中，APB抑制ON - 双极细胞，而不影响OFF - 双极细胞，且对其他视网膜神经元无直接影响（斯劳特和米勒，1981年、1985年）。如果APB在猫视网膜中有相同的作用方式，那么目前的结果表明ON通道对OFF - 神经节细胞存在甘氨酸能抑制性输入。
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