Zhejiang Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Zhejiang Hospital, Hangzhou, PR China.
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, PR China.
Commun Biol. 2024 Nov 8;7(1):1471. doi: 10.1038/s42003-024-07097-2.
Osteoporosis is a degenerative bone disease highly prevalent in older women, causing high morbidity and mortality rates. Fourteen kinds of fucoidan were isolated from Sargassum fusiforme through acid (named as SFS), alkaline (SFJ) and water (SFW). SFW was passed through an anion exchange column to obtain SFW-0, SFW-0.5 and SFW-2. SFW-0.5 and SFW-2 were degraded to obtain different sulfate group contents SFW-x-M/S/O (x for 0.5 or 2). We further confirmed SFW-0.5-O was the most effective fraction of SFW. SFW-0.5-O may have alternating backbones of (Gal)n and (Fuc)n, and the main sulfation may be at C2/C3 of the Fuc/Gal residues. SFW-0.5-O inhibition of OC differentiation was associated with IRF-8 signaling; meanwhile, SFW-0.5-O promoted osteoblast differentiation and bone mineral nodule formation. SFW-0.5-O also effectively ameliorated osteoporosis symptom caused by estrogen deprivation in vivo. We uncovered that the fucoidan active fraction SFW-0.5-O demonstrated effective bone protection, may be exploited for osteoporosis therapy.
骨质疏松症是一种退行性骨病，在老年女性中发病率很高，导致高发病率和死亡率。从马尾藻中分离出 14 种褐藻糖胶，分别通过酸（命名为 SFS）、碱（SFJ）和水（SFW）提取。SFW 通过阴离子交换柱得到 SFW-0、SFW-0.5 和 SFW-2。SFW-0.5 和 SFW-2 降解得到不同硫酸根含量的 SFW-x-M/S/O（x 为 0.5 或 2）。我们进一步证实 SFW-0.5-O 是 SFW 中最有效的部分。SFW-0.5-O 可能具有（Gal）n 和（Fuc）n 的交替骨架，主要的硫酸化可能发生在 Fuc/Gal 残基的 C2/C3 上。SFW-0.5-O 对 OC 分化的抑制与 IRF-8 信号有关；同时，SFW-0.5-O 促进成骨细胞分化和骨矿物质结节形成。SFW-0.5-O 还能有效改善体内雌激素缺乏引起的骨质疏松症症状。我们发现褐藻糖胶活性部分 SFW-0.5-O 具有有效的骨保护作用，可用于骨质疏松症的治疗。
